西北农业学报2025，Vol.34Issue(5)：826-839,14.DOI:10.7606/j.issn.1004-1389.2025.05.006
232份油菜种质萌发期耐NaCl胁迫评价及基因挖掘
NaCl Stress Resistance Evaluation of 232 Rapeseed Germplasms during Germination and Identification of Responsive Genes
摘要
Abstract
To screen NaCl-tolerant rapeseed germplasms and analyze the potential mechanisms of NaCl stress response at the transcriptional level,the NaCl resistance of 232 rapeseed germplasms dur-ing seed germination was evaluated.Ten NaCl-tolerant germplasms,including NWAFU114,and twelve NaCl-sensitive germplasms,including NWAFU99,were identified.Furthermore,we demonstra-ted that NWAFU114 enhanced NaCl tolerance during germination by promoting antioxidant enzyme activity and alleviating membrane damage.Using the identified NaCl-tolerant germplasms and NaCl-sensitive germplasms,a transcriptome analysis was performed with NaCl-treated germinating seeds cultured six hours under light condition after three days of stratification.A total of 12 135 differential-ly expressed genes(DEGs)were identified.Gene ontology(GO)analysis showed that among signifi-cantly enriched DEGs,many were involved in biological processes including responses triggered by ab-scisic acid(ABA),jasmonic acid(JA),oxidative stress,osmotic stress,and cell wall biogenesis,all of which are known to play an important role in plant stress responses.关键词
油菜/萌发期/NaCl胁迫/种质筛选/转录组Key words
Rapeseed/Germination/NaCl treatment/Germplasm screening/Transcriptome引用本文复制引用
陈佑芃,刘华,王鑫,张达,何双呈,蒋立希,刘子金,陈明训..232份油菜种质萌发期耐NaCl胁迫评价及基因挖掘[J].西北农业学报,2025,34(5):826-839,14.基金项目
国家科技创新2030项目(2022ZD04010) （2022ZD04010）
陕西省科技创新团队项目(2024RS-CXTD-69) （2024RS-CXTD-69）
杨凌种业创新中心项目(K3031122024). NationalScience and Technology Innovation 2030(No.2022ZD04010) （K3031122024）
Shaanxi Pro-vincial Science and Technology Innovation Team(No.2024RS-CXTD-69) （No.2024RS-CXTD-69）
Yangling Seed Industry In-novation Center(No.K3031122024). （No.K3031122024）
