西北农业学报2025，Vol.34Issue(5)：840-851,12.DOI:10.7606/j.issn.1004-1389.2025.05.007
库尔勒香梨不同品质果实代谢组差异
Metabolite Differences between Rough-skinned and Normal Korla Pears
摘要
Abstract
This studyinvestigates metabolite difference between rough-skinned and normal Korla pears during the ripening stage of Korla fragrant pears to enhance its quality,and to provide a theoretical basis for improving fruit quality and promoting the development of Korla fragrant pear products.Differences in fruit quality were distinguished through visual and physiological assessments.The me-tabolites were analyzed using ultra-high performance liquid chromatography tandem mass spectrome-try(UPLC).The data were processed by Principal Component Analysis(PCA),Orthogonal Partial Least Squares Discriminant Analysis(OPLS-DA),and clustering analysis.The combination of fold change and the OPLS-DA model VIP score were used to identify differential metabolites.A total of 815 metabolites were detected in Korla fragrant pear,which were categorized into 11 groups,inclu-ding flavonoids,phenolic acids,lipids,and terpenes,among these,flavonoids,phenolic acids,and lipids accounted for 52％of the total metabolites.A total of 163 different metabolites were identified in fruits of different quality.In rough-skinned pears,53 metabolites,including amino acids(8/9),or-ganic acids(8/9),and alkaloids(5/6),were found to be abundant and had high concentrations.In normal pears,114 metabolites,including flavonoids(56/64),phenolic acids(26/32),lignans,couma-rins(6/7),tannins(5/5),and quinones(2/2),were abundant.KEGG pathway enrichment analysis revealed that the differential metabolites were distributed in 51 metabolic pathways,with significant enrichment in flavonoid and flavonol metabolism,as well as flavonoid metabolism(P＜0.05).In nor-mal pears,eight types of flavonoids and flavonols were found in higher concentrations,while in rough-skinned pears,four types of alkaloids were present in higher concentrations,mainly synthe-sized through the metabolic pathways of arginine and proline.These findings suggest that the differ-ence in the quality of Korla fragrant pears may be related to the metabolism of flavonols and fla-vonoids,and synthesis pathways of arginine and proline.关键词
库尔勒香梨/代谢组/品质差异Key words
Korla fragrant pear/Metabolome/Different quality引用本文复制引用
李文慧,佟珂,樊国全,张婷婷,宿明洁,王绍鹏..库尔勒香梨不同品质果实代谢组差异[J].西北农业学报,2025,34(5):840-851,12.基金项目
新疆林果产业技术体系(XJLGCYJSTX05-2024-18) （XJLGCYJSTX05-2024-18）
自治区财政林草专项资金(种苗培养补助)国家物种保护项目(19190169). Xinjiang Forestry and Fruit Industry Technology System(No.XJLGCYJSTX05-2024-18) （种苗培养补助）
Special Funds for Forestry and Grass Development in the Xinjiang Uygur Autonomous Re-gion(Seedling Cultivation Subsidy)and National Species Conservation Project(No.19190169). （Seedling Cultivation Subsidy）
