西北农业学报2025，Vol.34Issue(5)：868-875,8.DOI:10.7606/j.issn.1004-1389.2025.05.010
辣椒粕多糖提取与分离及抑菌活性研究
Extraction,Isolation,and Antimicrobial Activity of Polysaccharides from a Chili Meal
摘要
Abstract
The study aimed to extract the different polysaccharide components from the waste chili meal and explore the monosaccharide compositions and antibacterial activities.The chili meal polysac-charide(CMP)was extracted using water extraction,followed by graded alcohol precipitation with ethanol concentrations of 40％,60％,80％,and 90％,obtaining CMP40,CMP60,CMP80,and CMP90.Afterward,the molecular mass of the CMP were determined by high-performance gel perme-ation chromatography.Monosaccharide compositions were determined using high-performance liquid chromatography.Finally,the growth of Escherichia coli,Salmonella,Staphylococcus aureus,and Lactobacillus bacteria was evaluated using the absorbance method.The results showed that the yields of the CMPs were 22.452％,5.000％,7.197％,and 0.664％,respectively.The CMP40 was mainly composed of macromolecular polysaccharides with a homogeneous distribution and a molecular mass(Mw)of 78.862 ku.The CMP60 was mainly composed of the homogeneous distribution polysaccha-ride with Mw of 17.785 ku.The CMP80 was composed of four polysaccharide molecules with Mw of 1.615-23.384 ku.The CMP90 was composed of two small-molecule polysaccharides with Mw of 2.617 ku and 1.642 ku.In addition,the monosaccharide composition of the CMP40 mainly consisted of galacturonic acid(69.397％)and glucose(18.009％).The monosaccharide composition of the CMP60 mainly consisted of galacturonic acid(29.037％),glucose(32.716％),and galactose(17.989％).The monosaccharide composition of the CMP80 mainly consisted of galacturonic acid(29.037％),glucose(32.716％),galactose(17.989％),and arabinose(16.467％).The monosac-charide composition of the CMP90 mainly consisted of glucose(80.362％).The in vitro bacteriostatic tests showed significant inhibitory effects of all CMP components on Escherichia coli,Salmonella,Staphylococcus aureus,and Lactobacillus(P＜0.05).The inhibitory effects increased with polysac-charide concentration,with CMP80 exhibiting the most pronounced antibacterial activity.The study provides an effective method for the high-value utilization of chili meal.关键词
辣椒粕/多糖/分子质量/单糖组成/抑菌活性Key words
Chili meal/Polysaccharide/Molecular mass/Monosaccharide composition/Antibacterial activity引用本文复制引用
李珂珂,岳自鹏,赫英辉,石达,翟天翔,赵酉辰,霍书英,秦鹏,李树鹏..辣椒粕多糖提取与分离及抑菌活性研究[J].西北农业学报,2025,34(5):868-875,8.基金项目
河北省自然科学基金(C2022204237) （C2022204237）
河北省现代农业产业技术体系专项(HBCT2024240207,HBCT2024250206). Natural Science Foundation of Hebei Province(No.C2022204237) （HBCT2024240207,HBCT2024250206）
Special Funds for Modern Agricultural Industry Technology System in Hebei Province(No.HBCT2024240207,No.HBCT2024250206). （No.HBCT2024240207,No.HBCT2024250206）
