烟草科技2025，Vol.58Issue(5)：9-18,25,11.
烟叶葡阿拉伯半乳聚糖的提取、纯化和结构解析
A novel glucoarabinogalactan from tobacco leaves:Extraction,purification and structural characterization
摘要
Abstract
To promote value-added transformation and utilization of tobacco resources,a hot water extraction method was used to extract polysaccharides from low-quality tobacco leaves.A novel glucoarabinogalactan,named WTLP2b,was isolated and purified by ion exchange chromatography and gel column chromatography.The structure of the WTLP2b sample was characterized using various analytical techniques,including ion chromatography,gel filtration chromatography,gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy.The results showed that:1)The WTLP2b obtained consisted of galactose(55％),arabinose(33％)and glucose(12％),with a molecular weight of 62.0 kDa.Methylation derivation and NMR analysis revealed that WTLP2b's main chain was composed of →6)-β-D-Galp-(1 →,while the side chains consisted of β-D-Galp-(1→4)-β-D-Galp-(1→,α-L-Araf-(1→4)-β-D-Glcp-(1→,and α-L-Araf-(1→5)-α-L-Araf-(1→,connected to the main chain via O-3 bond of →6)-β-D-Galp-(1→.2)The WTLP2b sample mainly showed a sheet-like structure with aggregated morphology.3)The thermal stability of the WTLP2b sample was relatively good.The results of this study show that tobacco leaves can serve as a natural source of glucoarabinogalactan.关键词
烟叶/多糖/葡阿拉伯半乳聚糖/提取/纯化/结构解析Key words
Tobacco leaf/Polysaccharide/Glucoarabinogalactan/Extraction/Purification/Structural characterization分类
轻工业引用本文复制引用
赵妍,张建平,蓝洪桥,黄华发,黄延俊,李巧灵,刘泽春,谢卫..烟叶葡阿拉伯半乳聚糖的提取、纯化和结构解析[J].烟草科技,2025,58(5):9-18,25,11.基金项目
福建中烟工业有限责任公司博士后项目"烟草果胶的结构分析及其热解研究"(FJZYKJJH2023BH002). （FJZYKJJH2023BH002）
