烟草科技2025，Vol.58Issue(5)：26-33,8.
非生物逆境胁迫对苗期烟草NtGGPPS家族基因表达及西柏三烯二醇含量的影响
Effects of abiotic stresses on expression of NtGGPPS family genes and cembratriene-diol contents in tobacco seedlings
摘要
Abstract
To investigate the mechanism of abiotic stresses on the accumulation of cembratriene-diol in tobacco seedlings,four abiotic stress treatments including low temperature,high temperature,drought and salt stresses were set up and followed by the analysis of the expression patterns of key enzyme GGPPS family members in the terpene synthesis pathway and the variation of cembratriene-diol content(mass fraction)in tobacco seedlings under these four abiotic stress conditions.The results showed that the expression of NtGGPPS family genes was significantly down-regulated under both the low and high temperature stresses,with the lowest expression level observed at 42 ℃.Drought stress also inhibited the expression of NtGGPPS family genes,and the expression of NtGGPPS1,NtGGPPS2 and NtGGPPS4 genes was significantly inhibited by salt stress.Conversely,the high temperature,drought and salt stresses induced the expression of the NtCYP71D16 gene.The cembratriene-diol content in tobacco seedlings was significantly increased under the high temperature,drought and salt stresses.In conclusion,the expression patterns of NtGGPPS family members and the cembratriene-diol synthase gene NtCYP71D16 as well as the accumulation of cembratriene-diol in tobacco seedlings were changed under the abiotic stress conditions.关键词
烟草/NtGGPPS基因/盐碱地/非生物逆境胁迫/高温/低温/干旱/盐Key words
Tobacco/NtGGPPS gene/Saline-alkali soil/Abiotic stress/High temperature/Low temperature/Drought/Salt分类
轻工业引用本文复制引用
孟晨,鲁雪莉,朱海,马斯琦,李义强,张洪博,徐宗昌..非生物逆境胁迫对苗期烟草NtGGPPS家族基因表达及西柏三烯二醇含量的影响[J].烟草科技,2025,58(5):26-33,8.基金项目
国家重点研发青年科学家项目"盐碱地适生多样性种植协同改土抗逆机制与调控技术研究"(2023YFD1901900) （2023YFD1901900）
中国农业科学院科技创新工程项目"基于代谢流调控及C-O键构建的萜烯成分生物合成"(ASTIP-TRIC-ZD03)、中国农业科学院科技创新工程项目(ASTIP-TRIC06)、中国农业科学院科技创新工程-中国农业科学院重大科技任务项目"盐碱地分区改良和综合利用技术集成示范"(CAAS-ZDRW202407). （ASTIP-TRIC-ZD03）
