烟草科技2025，Vol.58Issue(5)：34-45,12.
典型香型产区上部烟叶成熟过程中代谢物差异分析
Metabolite differences in upper tobacco leaves of flue-cured tobacco of typical flavor types grown in different areas during maturation
摘要
Abstract
To clarify the material transformation patterns and metabolite differences in upper tobacco leaves of flue-cured tobacco from different flavor type growing areas during the maturation process,tobacco leaves from three regions including Henan(robust flavor type),Guizhou(medium flavor type)and Yunnan(fresh flavor type)were selected as research subjects.The upper tobacco leaf samples were collected every 7 days after topping and analyzed by GC-MS in combination with Orthogonal Partial Least Squares-Discriminant Analysis(OPLS-DA)and Hierarchical Cluster Analysis(HCA).The results indicated that the ripening periods for the upper tobacco leaves of the robust,medium and fresh flavor types were 80,52 and 45 days after topping,respectively,with the robust flavor type leaves ripening significantly longer in the field.Based on the metabolite composition,the maturation process of the upper tobacco leaves could be roughly divided into three stages,namely early,middle and late maturation.Analysis of differential metabolites at the three stages of maturation showed that 76,63 and 44 differential metabolites were identified for the leaves of robust,medium and fresh flavor types,respectively,and the key metabolic pathways involved were 12,17 and 10 for the leaves of three flavor types.The core metabolic pathways common to all three flavor type leaves included galactose metabolism,fructose and mannose metabolism,alanine,aspartate and glutamate metabolism,glutathione metabolism,pentose phosphate pathway,and glycine,serine and threonine metabolism.In addition,anatabine,phytol and scopolamine was considered to be the key metabolites during the maturation of upper tobacco leaves of the robust flavor type;alpha-tocopherol could be the key metabolite for upper tobacco leaves of the medium flavor type;and D-rhamnose,scopolamine and L-lysine were the key metabolites for upper tobacco leaves of the fresh flavor type.关键词
上部烟叶/香型/代谢组学/差异代谢物/代谢通路Key words
Upper tobacco leaf/Flavor type/Metabolomics/Differential metabolite/Metabolic pathway分类
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金国慧,刘建利,王建伟,黄晓东,刘相甫,王桂瑶,梁太波,吴宝建,张艳玲..典型香型产区上部烟叶成熟过程中代谢物差异分析[J].烟草科技,2025,58(5):34-45,12.基金项目
中国烟草总公司重点研发项目"品牌导向型高可用性上部烟叶开发与高效利用技术研究"(110202102035). （110202102035）
