中国草地学报2025，Vol.47Issue(5)：34-45,12.DOI:10.16742/j.zgcdxb.20240340
329份冰草材料种子表型性状遗传多样性分析与综合评价
Genetic Diversity and Phenotypic Traits Evaluation of 329 Agropyron cristatum Germplasm Resources
摘要
Abstract
This study employed multivariate statistical methods,including analysis of variance(ANOVA),principal component analysis(PCA),correlation analysis,hierarchical cluster analysis,and membership function evaluation,to comprehensively analyze and evaluate five seed morphological traits of 329 wildrye(Agropyron cristatum)accessions conserved in the National Crop GeneBank.The objective was to establish a theoretical framework for the evaluation and innovative utilization of A.cristatum(L.)Gaertn germplasm resources.The results showed that among the five phenotypic traits examined,thousand-seed weight exhibited the highest genetic variation,with a genetic diversity index of 1.880 and a coefficient of variation of 24.56%.The average genetic diversity index for all five traits was 1.752,and the average coefficient of variation of 14.06%.PCA extracted three principal components,explaining 98.750%of the trait variation,with the first three main components contributing 65.555%,26.162%,and 7.034%respectively,effectively preserving the core genetic information of seed traits.Correlation analysis demonstrated extremely significant positive correlations between seed length with the other four phenotypic traits(P<0.01).Additionally,significant positive correlations were observed between seed width and both thousand-seed weight and single-seed area(P<0.01),between thousand-seed weight and seed length-width ratio,as well as between seed length-width ratio and single-seed area.Hierarchical clus-ter analysis categorized the accessions into four groups:Group Ⅰ(284 accessions,moderate),Group Ⅱ(41 accessions,small-grained),and Groups Ⅲ and Ⅳ(two high-quality unique accessions each).Compre-hensive evaluation using the membership function identified B-288,B-243,B-233,and B-250 accessions with excellent performance in seed length,seed width,single-seed area,and thousand-seed weight.In con-trast,B-152,B-143,B-128,B-149,B-141,and B-153 accessions exhibited relatively weak performance across multiple traits.In conclusion,seed length,single-seed area and thousand-seed weight were identified as the key morphological indicators contributing to the genetic diversity of A.cristatum germplasm.关键词
冰草/种子表型性状/主成分分析/聚类分析/隶属函数分析Key words
Agropyron cristatum(L.)Gaertn/Seed phenotypic traits/Principal component analysis/Cluster analysis/Membership function analysis分类
农业科技引用本文复制引用
李悦煊,黄雪琦,白小明,辛霞..329份冰草材料种子表型性状遗传多样性分析与综合评价[J].中国草地学报,2025,47(5):34-45,12.基金项目
中国农业科学院科技创新工程(CAAS-ASTIP-2024-ICS01) （CAAS-ASTIP-2024-ICS01）
