中国草地学报2025，Vol.47Issue(5)：89-101,13.DOI:10.16742/j.zgcdxb.20240227
不同非生物胁迫对蒺藜苜蓿生长及表观遗传修饰特征的影响
Effects of Various Abiotic Stresses on Growth and Characteristics of Epigenetic Modifications in Medicago truncatula
摘要
Abstract
Epigenetics plays a pivotal role in the adaptation to fluctuating external environmental condi-tions.Abiotic stresses alter the epigenetic modification status of plants,which in turn regulate stress responses of plants.This study presented a comprehensive analysis of the morphological,physiological,and epigenetic dynamics in Medicago truncatula when subjected to salinity,drought,heat,and cold stresses.The interplay between epigenetic modifications with the morphological and physiological traits of Medicago truncatula under stress conditions were explored by correlation analysis.Results revealed that these abiotic stresses significantly inhibited the growth and physiological status of Medicago truncatula,in which reduction in leaf area(except under low temperature),the weight of leaves(dry),stems(dry)and above ground fresh(dry)was significantly reduced relative water content,and total chlorophyll content,and increase in relative electrical conductivity,soluble sugar,and total antioxidant capacity were observed(P<0.05).Meanwhile,epigenetics was involved in the regulatory processes of Medicago truncatula under stress conditions,though the regulatory mechanisms among various epigenetic modifications were dif-ferent.Under all four stress conditions,DNA methylation levels significantly increased,while acetylated histone content reduced.Methylated histone content increased only under salt stress,with decreases under the other three stresses.Histones ubiquitinylation was risen under salt and low-temperature stress,whereas high-temperature stress reduced this content(P<0.05),also with no significant changes under drought stress.Correlation analysis further indicated a positive correlation between N-6-methyladenosine content and acetylated histones with leaf morphological indicators,such as leaf fresh weight,leaf dry weight,and leaf area,except in acetylated histones content with leaf area.Conversely,this two parameters were negatively correlated with physiological indicators such as relative electrical conductivity,soluble sug-ars,and total antioxidant capacity,except between N-6-methyladenosine content with relative electrical con-ductivity.This research provides preliminary evidence in adaptive characteristics of Medicago truncatula in response to various abiotic stresses from an epigenetic perspective and lays a foundational theoretical frame-work for the epigenetic molecular breeding of legume forage grasses to enhance stress tolerance.关键词
蒺藜苜蓿/非生物胁迫/DNA甲基化/组蛋白修饰/表观遗传Key words
Medicago truncatula/Abiotic stress/DNA methylation/Histone modification/Epigenetic分类
农业科技引用本文复制引用
赵媛媛,徐成体,王伟,蒲小剑,魏希杰,张俊超..不同非生物胁迫对蒺藜苜蓿生长及表观遗传修饰特征的影响[J].中国草地学报,2025,47(5):89-101,13.基金项目
青海大学青年科研基金(2023-QNY-6) （2023-QNY-6）
省部共建三江源生态与高原农牧业国家重点实验室自主课题(2024-ZZ-16) （2024-ZZ-16）
三江源区高寒草地生态教育部重点实验室开放基金(2023-SJY-KF-03) （2023-SJY-KF-03）
国家自然科学基金项目(32201452) （32201452）
