中国草食动物科学2025，Vol.45Issue(3)：29-38,10.DOI:10.3969/j.issn.2095-3887.2024.00.126
基于网络药理学及分子对接探究马齿苋缓解肉兔肠炎的作用机制
Exploring the Mechanism of Purslane in Relieving Enteritis of Meat Rabbits Based on Network Pharmacology and Molecular Docking
摘要
Abstract
The aim of this study was to explore the mechanism of purslane in alleviating enteritis of meat rabbits using network pharmacology and molecular docking techniques.The main active ingredients and related targets of purslane were identified using the TCMSP database,and the disease targets of rabbit enteritis were searched for in the Gene-Cards database.Interaction between the intersecting targets of the two were analyzed by STRING,and the core targets were screened.GO and KEGG enrichment analysis were performed to obtain the relevant signaling pathways of Portu-laca oleracea's anti-inflammatory effects.The top 5 active ingredients with the highest moderate values in Portulaca oleracea were selected,then validated by molecular docking with the selected core targets using the AutoDock Vina plu-gin in AutoDock software.The results showed that the main active ingredients of Portulaca oleracea were 10 types,in-cluding β-carotene,β-sitosterol,luteolin,quercetin,etc.,with a total of 267 corresponding targets and 52 core tar-gets.The KEGG enrichment analysis revealed that the core targets were closely related to pathways related to inflam-mation,cancer,and viral diseases.The main active ingredients of Portulaca oleracea,such as quercetin,luteolin,kaemp-ferol,5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)chroman-4-one,arachidonic acid,had strong binding activity with the core targets PLK1,SYK,PLA2G4A,MPO and GUSB.In conclusion,purslane may act on major signaling path-ways such as PI3K Akt,mTOR,NF-κB through core targets such as PLK1,SYK,PLA2G4A,MPO and GUSB,thereby af-fecting physiological processes such as inflammatory response,cell apoptosis,and cell proliferation and differentiation in enteritis,and play a role in relieving enteritis.关键词
马齿苋/肉兔/肠炎/网络药理学/分子对接Key words
purslane/meat rabbit/enteritis/network pharmacology/molecular docking分类
畜牧业引用本文复制引用
谢浦航,张子儒,唐晓琴,张贝贝,邱彦博,佘木子,孙秀柱..基于网络药理学及分子对接探究马齿苋缓解肉兔肠炎的作用机制[J].中国草食动物科学,2025,45(3):29-38,10.基金项目
农业农村部"家兔肉质性状表型鉴定"(19240889) （19240889）
