中国康复2025，Vol.40Issue(5)：259-264,6.DOI:10.3870/zgkf.2025.05.001
经颅直流电刺激联合穴位针刺对脑梗死大鼠学习记忆能力、神经细胞自噬及脑组织BDNF、NGF表达的影响
摘要
Abstract
Objective:To explore the impacts of transcranial direct-current stimulation(tDCS)combined with acu-point acupuncture on the learning and memory ability,nerve cell autophagy,and the expression levels of brain-de-rived neurotrophic factor(BDNF)and nerve growth factor(NGF)in ischemic stroke rats.Methods:In total,45 healthy male SPF SD rats were selected.Among them,35 rats were used to establish a stroke model.Totally,30 rats were successfully modeled and then divided into the ischemic stroke group,the tDCS group,and the combined group,with 10 rats in each group.The remaining 10 rats served as the control group.The control group and the is-chemic stroke group received no intervention.The tDCS group was treated with tDCS once a day for 14 days.The combined group received acupoint acupuncture intervention in addition to tDCS treatment once a day for 14 days.The Morris water maze test was employed to assess the learning and memory ability.Pathological changes were ob-served by hematoxylin-eosin(HE)staining.The ex-pression of BDNF and NGF was detected by immuno-histochemistry.Western blotting was used to detect the nerve cell autophagy-related proteins Beclin1 and LC3,and reverse-transcription polymerase chain reaction(RT-PCR)was applied to detect Beclin1 and LC3 mR-NA.Results:Compared with the control group,the escape latency,orientation angle,Beclin1 and LC3 protein ex-pression level increased,while the residence time in the third quadrant(RTQ),cross-platform frequency,and BD-NF and NGF content decreased in the ischemic stroke group(P＜0.05).Compared with the ischemic stroke group,the escape latency,orientation angle,Beclin1 and LC3 protein expression level decreased,while the RTQ,cross-platform frequency,and BDNF and NGF content increased in the tDCS group(P＜0.05).Compared with the tDCS group,the escape latency,orientation angle,Beclin1 and LC3 protein expression level further decreased,while the RTQ,cross-platform frequency,and BDNF and NGF content further increased in the combined group(P＜0.05).HE staining results demonstrated that the brain tissue of rats in the control group was intact,while that in the is-chemic stroke group was severely damaged,with a large number of cells showing disordered arrangement and vacuo-lar degeneration.The pathological conditions in both the tDCS group and the combined group were alleviated,and the morphology of nerve cell nuclei and nucleoli gradually became clear and complete,with the combined group showing the most significant improvement.Conclusion:The tDCS combined with acupoint acupuncture exerts a fa-vorable effect on the treatment of stroke.The mechanism may be associated with enhancing learning and memory a-bility,improving nerve cell autophagy,and regulating the levels of BDNF and NGF.关键词
脑梗死/经颅直流电刺激/穴位针刺/学习记忆/神经细胞自噬/脑源性神经营养因子/脑神经生长因子Key words
ischemic stroke/transcranial direct-current stimulation/acupoint acupuncture/learning and memory/nerve cell autophagy/brain-derived neurotrophic factor/nerve growth factor分类
医药卫生引用本文复制引用
张贤,张保,柏中喜,刘迪,储小宁,郑苏..经颅直流电刺激联合穴位针刺对脑梗死大鼠学习记忆能力、神经细胞自噬及脑组织BDNF、NGF表达的影响[J].中国康复,2025,40(5):259-264,6.基金项目
中医药科研立项项目(青年人才项目)(ZY2021Q015) （青年人才项目）
