中国草食动物科学2025，Vol.45Issue(3)：106-112,7.DOI:10.3969/j.issn.2095-3887.2024.00.090
甘肃临洮地区绵羊捻转血矛线虫病流行情况及系统发育分析
Prevalence Status and Phylogenetic Analysis on Haemonchus Contortus of Ovine in Lintao County,Gansu Province
摘要
Abstract
The aim of this study was to investigate the prevalence status and the genetic variation of Haemonchus con-tortus in ovine in Lintao,Gansu province.416 sheep fecal samples were collected from 18 townships in Lintao county using the saturated salt water floating method.ITS-2 gene of Hemoncus contortus was amplified and sequenced by PCR.The base content of ITS-2 sequence was analyzed by DNAStar7.1 software.Moreover,the ITS-2 phylogenetic tree was constructed to analyze the genetic and variation situation of Haemonchus contortus for sheep in Lintao county by using MEGA-X software based on the ITS-2 sequences of 21 isolates from different countries and regions disclosed in GenBank database.The results showed that 126 of the 416 samples were positive for Hemoncus contortus(30.29%);the infection rates in summer and autumn seasons and in winter and spring seasons were 36.05%and 25.24%,respec-tively,with extremely significant differences(P<0.01);the infection rates of young sheep(0-8 months old)and adult sheep(over 9 months old)were 31.95%and 29.63%,respectively,with significant differences(P<0.05).The sequence analysis results showed that the ITS-2 sequences of 126 strains of Hemoncus contortus obtained in this study were all 232 bp in length,and the consistency was 100%,which was named Dingxi strain.The ITS-2 sequence of Dingxi strain showed 95.0%-100%sequence identity with that of Hemoncus contortus isolates from different countries and regions.Phylogenetic tree results showed that the ITS-2 sequence of Dingxi strain was clustered in one branch with those from India(GenBank No.OM334845.1),Chinese Guangdong(GenBank No.KM586652.1)and Ireland(GenBank No.JF680983.1),and they had a close genetic relationship.关键词
绵羊/捻转血矛线虫/ITS-2基因/系统进化分析Key words
ovine/Haemonchus contortus/ITS-2 gene/phylogenetic tree分类
农业科技引用本文复制引用
李永光,盖文燕,郑小红,吴耀辉,雒和静,王筠茹..甘肃临洮地区绵羊捻转血矛线虫病流行情况及系统发育分析[J].中国草食动物科学,2025,45(3):106-112,7.基金项目
定西市科技计划项目(DX2023BR01) （DX2023BR01）
甘肃省职业教育专业教学资源库建设项目 （）
家畜疫病病原生物学国家重点实验室(中国农业科学院兰州兽医研究所)开放课题(SKLVEB2021KFKT014) （中国农业科学院兰州兽医研究所）
