中国感染控制杂志2025，Vol.24Issue(5)：618-624,7.DOI:10.12138/j.issn.1671-9638.20252080
可可毛色二孢菌致真菌性角膜炎1例及文献回顾分析
Fungal keratitis caused by Lasiodiplodia theobromae:a case report and literature review
陈映 1李洪 1王会玉 1黄广定 1王琼瑛 1李小芳2
作者信息
- 1. 琼海市人民医院检验科,海南琼海 571400
- 2. 琼海市人民医院眼科,海南琼海 571400
- 折叠
摘要
Abstract
Objective To report the diagnosis and treatment processes of a fungal keratitis case caused by Lasio-diplodia theobromae(L.theobromae),and enhance the diagnosis and treatment experience on fungal keratitis caused by this rare pathogen.Methods Corneal scraping specimen from a patient with fungal keratitis was collec-ted.Gram-staining and fluorescence staining were conducted on specimen,followed with direct microscopic observa-tion and isolation culture.The strain was identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS)technology and targeted DNA sequencing.Antimicrobial susceptibility testing was conducted.Literatures were summarized and clinical data on fungal keratitis caused by this pathogen were sorted out.Results Septal fungal hyphae could be seen on the corneal scraping specimen microscopically.The strain was identified as L.theobromae by both MALDI-TOF MS and targeted DNA sequencing after culture.Antimicrobial susceptibility testing(microdilution method)showed that the minimal inhibitory concentrations(MICs)of ampho-tericin B,vorionazole,itraconazole,posaconazole,fluconazole,5-fluorocytosine,micafungin,caspofungin,and anidulafungin against this strain were 1,0.25,＞8,0.25,＞256,8,16,2,and 0.25 μg/mL,respectively.Pa-tient recovered well after antifungal treatment plan was adjusted according to antimicrobial susceptibility testing re-sults.Conclusion L.theobromae is a rare pathogen that causes fungal keratitis.Laboratory tests provide rapid mi-croscopic examination results,and take MALDI-TOF MS and targeted DNA sequencing identification techniques as effective means to detect rare pathogen.In vitro antimicrobial susceptibility testing result can provide reference for clinicians to correctly use antifungal agents for treatment of infection due to this pathogen.关键词
可可毛色二孢菌/真菌性角膜炎/病例报告Key words
Lasiodiplodia theobromae/fungal keratitis/case report分类
医药卫生引用本文复制引用
陈映,李洪,王会玉,黄广定,王琼瑛,李小芳..可可毛色二孢菌致真菌性角膜炎1例及文献回顾分析[J].中国感染控制杂志,2025,24(5):618-624,7.
