中国蔬菜Issue(5)：131-137,7.DOI:10.19928/j.cnki.1000-6346.2025.4015
叶面喷施2,4-表油菜素内酯对盐碱胁迫下菜薯幼苗根系生长及生理的影响
The Effect of Exogenous 2,4-epobrassinolide on Root Growth and Physiological Characteristics of Vegetably Potato Seedlings under Saline Alkali Stress
摘要
Abstract
This study investigated the effects of exogenous 2,4-epibrassinolide(EBR)on the growth and physiology of vegetably potato seedlings('Ecaishu No.10')under saline-alkali stress to establish a theoretical basis for applying EBR to alleviate stress.A pot experiment was conducted to evaluate the impacts of foliar spraying with different EBR concentrations under 300 mmol·L-1 saline-alkali stress on root vitality,photosynthetic parameters(Pn,Tr,andGs),chlorophyll fluorescence characteristics(Fo,Fm,Fv,Fv/Fo,and Fv/Fm),antioxidant enzyme activities(SOD,POD,and CAT),and osmoregulatory substance contents.Results showed that saline-alkali stress severely inhibited seedling growth:compared to the control(CK),root number,root activity,and root dry weight decreased by 46.97％,38.43％,and 47.46％,respectively;Pn,Tr,and Gs significantly declined;initial fluorescence(Fo)increased,while maximum fluorescence(Fm),variable fluorescence(Fv),PSⅡ potential activity(Fv/Fo),and maximum photochemical efficiency(Fv/Fm)decreased significantly.Malondialdehyde(MDA)content,relative electrolyte leakage,and antioxidant enzyme activities(SOD,POD,and CAT)were significantly elevated.Exogenous EBR effectively promoted root growth under stress:T3 treatment(1.25 μmol·L-1 EBR)exhibited the highest root number and root activity,increasing by 68.10％and 61.29％compared to T0(stress without EBR),while root dry weight peaked in T4(1.25 μmol·L-1 EBR)at 0.97 g per plant,with no significant difference between T3 and T4.Pn,Tr,and Gs reached maximum values under T3,increasing by 120.83％,134.02％,and 98.22％relative to T0.T3 also showed the highest proline content(35.01％increase),soluble protein content(25.70％),and soluble sugar content(33.01％)among all treatments.In conclusion,foliar application of 1.25 μmol·L-1 EBR effectively improved saline-alkali stress tolerance in potato seedlings by enhancing membrane stability,boosting photosynthetic efficiency,and mitigating stress-induced growth inhibitiot.关键词
2,4-表油菜素内酯(EBR)/盐碱胁迫/菜薯幼苗/光合特性/渗透调节物质Key words
EBR/saline alkali stress/vegetably potato seedlings/photosynthetic characteristics/permeation regulating substance引用本文复制引用
孙喜云,丁芳,刘广卿,韩玉璞,孟彦,孟超凡..叶面喷施2,4-表油菜素内酯对盐碱胁迫下菜薯幼苗根系生长及生理的影响[J].中国蔬菜,2025,(5):131-137,7.基金项目
河南省"四优四化"科技支撑项目(SYSH20230504009),商丘市农林科学院科技攻关项目(20240503003) （SYSH20230504009）
