中国老年保健医学2025，Vol.23Issue(2)：34-39,6.DOI:10.3969/j.issn.1672-2671.2025.02.007
Compound A9抑制蛛网膜下腔出血后谷氨酸诱导神经细胞铁死亡的研究
Compound A9 inhibits glutamate-induced ferroptosis in neurons following subarachnoid hemorrhage
摘要
Abstract
Objective To investigate whether glutamate induces ferroptosis in neurons following subarachnoid hemorrhage(SAH)and to explore the neuroprotective effect of Compound A9.Methods Eighty Sprague-Dawley rats were randomly divided into four cohorts:a sham-operated control group,an SAH model group,an SAH model+glutamate group,and an SAH model+glutamate+Compound A9 group.At 48 hours post-SAH,neurological function was assessed using a standardized scoring system,while the lev-els of glutathione(GSH)and lactate dehydrogenase(LDH)were quantified via enzyme-linked immunosorbent assay(ELISA).Western blot analysis was employed to determine the expression levels of 4-hydroxynonenal(4-HNE)and glutathione peroxidase 4(GPX4),and immunofluorescence staining was conducted to colocalize 4-HNE with Neun,a neuronal marker.Foot fault tests were performed on days 5,7,and 12 to evaluate motor coordination.Results Compound A9 mitigated glutamate-induced neurological deficits and motor function impairments post-SAH.It also reduced oxidative damage and lipid peroxidation induced by glutamate.Furthermore,Compound A9 suppressed glutamate-induced ferroptosis by upregulating GPX4 expression.Conclusion Glutamate induces ferropto-sis in neurons following SAH,and Compound A9 inhibits glutamate-induced ferroptosis by upregulating GPX4 expression.关键词
蛛网膜下腔出血/谷氨酸/铁死亡/神经保护/Compound A9Key words
subarachnoid hemorrhage/glutamate/ferroptosis/neuroprotection/Compound A9引用本文复制引用
朱良珍,唐强飞,王玮琦,周国庆,侯亚军..Compound A9抑制蛛网膜下腔出血后谷氨酸诱导神经细胞铁死亡的研究[J].中国老年保健医学,2025,23(2):34-39,6.基金项目
山东省医药卫生科技项目(编号:202303071381),山东省自然科学青年基金(编号:ZR2022QH169),山东省中医药科技项目(编号:M-2023057),泰安市科技创新发展项目(编号:2022NS151) （编号:202303071381）
