中国瓜菜2025，Vol.38Issue(5)：20-27,8.DOI:10.16861/j.cnki.zggc.2025.0033
甜瓜转基因株系表型性状差异的研究
Study on phenotypic differences of transgenic lines in melon
摘要
Abstract
In this study,the gene MELO3C002050 of melon was edited by CRISPR-Cas9 technology,aiming to explore the ploidy changes of transgenic melon and its effects on phenotype traits.The phenotype and gene editing of 51 positive seedlings showed that the fruits of 45 plants(type A)were significantly elongated,and the compound gene editing was ex-pected,and the gene editing was successful.6 plants(type B)showed abnormal phenotypes such as enlarged flower or-gans,shortened internodes,shortened fruits,and seed width,but no gene editing occurred.Flow cytometry showed that the DNA content of type B plants was doubled(tetraploid rate of 11.76％).The results of the study showed that during the genetic transformation of melon,it may lead to the spontaneous doubling of ploidy,which may cause phenotypic abnor-malities.Therefore,in the phenotypic evaluation of melon gene editing,ploidy detection is given priority to exclude poly-ploid interference,and it is particularly important to accurately evaluate the phenotype of gene-edited plants.关键词
甜瓜/转基因植株/多倍体/流式细胞仪Key words
Melon/Transgenic plants/Polyploidy/Flow cytometry分类
农业科技引用本文复制引用
刘秉雪,贺玉花,徐永阳,田小琴,张健,孔维虎,唐伶俐,赵光伟..甜瓜转基因株系表型性状差异的研究[J].中国瓜菜,2025,38(5):20-27,8.基金项目
中国农业科学院郑州果树研究所科技创新工程(CAAS-ASTIP-ZFRI-06-202503) （CAAS-ASTIP-ZFRI-06-202503）
国家现代农业产业技术体系(CARS-25-2025-G6) （CARS-25-2025-G6）
中国农业科学院科技创新工程(CAAS-ASTIP-2025-ZFRI) （CAAS-ASTIP-2025-ZFRI）
国家自然科学基金(32172581) （32172581）
河南省科技攻关计划项目(252102110298) （252102110298）
