中国瓜菜2025，Vol.38Issue(5)：37-48,12.DOI:10.16861/j.cnki.zggc.2025.0073
海南三亚地区豇豆枯萎病病原菌生物学测定及室内药剂筛选
The biological characteristics of pathogens cowpea wilt disease in Sanya,Hainan,and the screening of effective fungicides
摘要
Abstract
To clarify the pathogen species and biological characteristics of cowpea wilt disease in Sanya,Hainan province,and screen out suitable agents for its prevention and control,three representative pathogen strains were separated from diseased cowpea plant roots and stems.The pathogenicity was determined using the Koch-style rule,and the strains were identified through morphological features and genetic analysis of ITS,TEF-1α,and TUB gene sequences.The biological characteristics of pathogenic bacteria and the sensitivity determination of common fungicides were analyzed by mycelial growth rate assay.The pathogen was identified as Fusarium oxysporum f.sp.tracheiphilum based on combined results of the pathogenicity test(the pathogen only infects cowpea)and molecular identification.Biological tests revealed that the optimal conditions for the vegetative growth of strains were lactose and maltose as carbon sources,potassium nitrate and sodium nitrate as nitrogen sources,at temperature of 20-30℃,pH 7.0-10.0,and in complete darkness.The strains grew fastest on CDA medium,with a lethal temperature of 65-70℃.Furthermore,the inhibitory effects of ten agents,among which fungicide-prochloraz had the best antifungal activity,with EC50 values of 0.034 4 μg·mL-1.This study provides scien-tific evidence and theoretical guidance for diagnosing and effectively controlling cowpea wilt in Sanya,Hainan.关键词
豇豆/豇豆枯萎病/尖孢镰刀菌嗜导管专化型/生物学测定/药剂筛选Key words
Cowpea/Cowpea wilt diseases/Fusarium oxysporum f.sp.tracheiphilum/Biological identification/Fungi-cide screening分类
农业科技引用本文复制引用
易剑锋,许沛冬,陈迪,赵廷昌,易克贤,林岸,谢丙炎,赵文军..海南三亚地区豇豆枯萎病病原菌生物学测定及室内药剂筛选[J].中国瓜菜,2025,38(5):37-48,12.基金项目
海南省自然科学基金青年项目(324QN365) （324QN365）
三亚崖州湾科技城"崖州湾"菁英人才科技专项项目(SCKJ-JYRC-2023-44) （SCKJ-JYRC-2023-44）
海南省"南海新星"科技创新人才平台项目(NHXXRCXM202358) （NHXXRCXM202358）
