中国瓜菜2025，Vol.38Issue(5)：55-62,8.DOI:10.16861/j.cnki.zggc.2024.0766
大白菜新品种豫白CR3的纯度分子鉴定
Molecular identification of the purity of the new Chinese cabbage variety Yubai CR3
摘要
Abstract
The identification of seed purity is imperative to ensure the quality and yield of crops.Conventional field identi-fication methods are inherently limited,and in recent years,molecular marker technologies have been employed in seed purity identification to enhance the accuracy and efficiency of the process.The present study employed three methods-KASP marker,InDel marker and S haplotype type marker based on self-incompatibility to identify the purity of seeds from F1 Chinese cabbage hybrid Yubai CR3.The results demonstrated that among the 188 samples of Yubai CR3,the aforementioned three markers consistently identified two pseudo-hybrids:A4(self-cross of the parent Y636-9)and G2(self-cross of the parent 14CR345M-3).The remaining 186 samples were identified as true Yubai CR3 hybrids,possess-ing alleles of both parents and their purity was 98.94％,thus verifying the consistency and accuracy of the three methods.The study demonstrated that the combined application of multiple molecular marker technologies significantly enhanced the accuracy of seed purity identification,provided substantial technical support for the promotion of Yubai CR3 Chinese cabbage,and also furnished a robust reference for the purity testing of other agricultural seeds.关键词
大白菜/纯度鉴定/豫白CR3/分子标记Key words
Chinese cabbage/Purity identification/Yubai CR3/Molecular marker分类
农业科技引用本文复制引用
寇欣怡,原玉香,孙守如,魏小春,朱磊,杨双娟,张文静,苏贺楠,赵艳艳,王志勇,张晓伟..大白菜新品种豫白CR3的纯度分子鉴定[J].中国瓜菜,2025,38(5):55-62,8.基金项目
中原科技创新领军人才(244200510041) （244200510041）
河南省科技研发计划联合基金优势学科重点项目(232301420024) （232301420024）
国家自然科学基金(32202485) （32202485）
