中国医院用药评价与分析2025，Vol.25Issue(5)：513-516,4.DOI:10.14009/j.issn.1672-2124.2025.05.001
基于噬菌体展示技术的替雷利珠单抗特异性结合肽筛选鉴定及其应用研究
Screening,Identification and Application of Tislelizumab-Specific Binding Peptides Based on Bacteriophage Display Technology
摘要
Abstract
OBJECTIVE:To develop an efficient and low-cost point-of-care assay for blood concentration monitoring of the immune checkpoint inhibitor tislelizumab,to address the limitations of existing assays(enzyme linked immunosorbent assay,liquid chromatography-tandem mass spectrometry)in clinical applications,and to promote the development of individualized precision therapy.METHODS:Tislelizumab-specific binding cyclic peptide(CP1)was obtained by bacteriophage display technology and three rounds of biological panning,and the optimized peptide CP1.1 was immobilized on the surface of gold nanoparticles through thiol-gold coordination,and an ultraviolet sensor(CP1.1@AuNPs)based on the aggregation/dispersion of gold nanoparticles produced by color change was constructed.RESULTS:The specific peptides with high affinity(KD=1.01 μmol/L)and no immunoglobulin G cross-reactivity were screened,and the sensor could quickly detect tislelizumab through the ultraviolet signal change of peptide-antibody binding induced by AuNPs aggregation,the sensitivity was significantly better than that of traditional methods.CONCLUSIONS:This study provides a new solution for the monitoring of monoclonal antibody drug concentration,and its peptide recognition element can be extended to detection platforms such as lateral flow chromatography,which lays a foundation for dynamic monitoring and individualized administration of immunotherapy drugs,and its practical application value needs to be verified through clinical samples in the future.关键词
替雷利珠单抗/噬菌体展示/多肽/金纳米颗粒/浓度检测Key words
Tislelizumab/Bacteriophage display/Peptides/Gold nanoparticles/Concentration detection分类
药学引用本文复制引用
陈雨,樊芳,袁智龙,张雨欣,王卓..基于噬菌体展示技术的替雷利珠单抗特异性结合肽筛选鉴定及其应用研究[J].中国医院用药评价与分析,2025,25(5):513-516,4.基金项目
国家自然科学基金资助项目(No.82204339) （No.82204339）
长海医院"234学科攀登计划"(No.2019YXK012) （No.2019YXK012）
