中国瓜菜2025，Vol.38Issue(5)：81-90,10.DOI:10.16861/j.cnki.zggc.2024.0576
响应面法优化平菇液体菌种培养体系及与固体菌种栽培对比
Optimization of Pleurotus ostreatus liquid culture medium using re-sponse surface methodology and its cultivation comparison with solid spawn
摘要
Abstract
To improve the mycelium biomass of Pleurotus ostreatus liquid spawn,a single-factor experiment was conduct-ed to analyze the effects of 5 carbon sources and 5 nitrogen sources on mycelial growth.Response surface methodology was then used to further optimize the liquid culture medium system for Pleurotus ostreatus,and the optimized liquid cul-ture was compared with solid spawn for cultivation.The results showed that the optimal formula of Pleurotus ostreatus liquid spawn cultivation system was soluble starch 50 g·L-1,soybean meal 34 g·L-1,MgSO4 1.25 g·L-1,KH2PO4 2 g·L-1 and vitamin B1 20 mg·L-1.The yield of mycelium biomass under this formula was 35.97 g·L-1,which was about 3.5 times higher than before optimization.The cultivation trial of Pleurotus ostreatus revealed that the optimized liquid spawn culture system shortened the production cycle to 34 days,which was 21 days shorter than that of the solid strain.In summary,the liquid culture medium formulation optimized by response surface methodology can produce high-quality Pleurotus ostreatus liquid spawn.关键词
平菇/液体菌种/响应面法/栽培对比Key words
Pleurotus ostreatus/Liquid spawn/Response surface method/Cultivation comparison分类
农业科技引用本文复制引用
王敬文,闻亚美,王奇,刘元栋,孔维丽..响应面法优化平菇液体菌种培养体系及与固体菌种栽培对比[J].中国瓜菜,2025,38(5):81-90,10.基金项目
河南省现代农业产业技术体系建设专项(HARS-22-08-Z4) （HARS-22-08-Z4）
