中国医院用药评价与分析2025，Vol.25Issue(5)：539-544,6.DOI:10.14009/j.issn.1672-2124.2025.05.006
银纳米颗粒-S-亚硝基谷胱甘肽胶囊治疗辐射-诱导肠炎小鼠模型肠道感染的活性研究
Activity of Silver-S-Nitrosoglutathione Nanoparticles Capsules in the Treatment of Intestinal Infection in Mouse Model with Radiation-Induced Enteritis
摘要
Abstract
OBJECTIVE:To probe into the activity of silver-S-nitrosoglutathione nanoparticles(Ag-GSNO NPs)capsules in the treatment of intestinal infection in mouse model with radiation-induced enteritis.METHODS:GSNO was synthesized and Ag-GSNO NPs capsules were prepared.(1)The antibacterial ability of GSNO powder or Ag-GSNO NPs powder against Staphylococcus aureus was determined by colony forming unit counting,the in vitro cytotoxicity of GSNO powder or Ag-GSNO NPs powder on human colon adenocarcinoma cells was determined by the cell proliferative capacity assay.The experiments were divided into the Con group,GSNO group and Ag-GSNO NPs group.(2)The ability of GSNO powder or Ag-GSNO NPs powder to produce nitric oxide(NO)was analyzed by NO analyzer,the experiments were grouped into GSNO group and Ag-GSNO NPs group.(3)A total of 40 SPF-grade C57BL/6 male mice aged 6 to 8 weeks were randomly divided into Control group(mice were given sham radiation),Model group(radiation-induced enterocolitis mouse model was established),Model+GSNO group(modeled mice were given GSNO capsules 7 μg by oral tube feeding right after irradiation,and giving GSNO capsules 7 μg by oral tube feeding for the next 3 consecutive days)and Model+Ag-GSNO NPs group(modeled mice were given Ag-GSNO NPs capsules 10 μg by oral tube feeding right after irradiation,and given Ag-GSNO NPs capsules 10 μg by oral tube feeding for the next 3 consecutive days).Mice weight was monitored from the day of modeling(day 0)to the fourth day.On the fourth day,mice were executed by cervical dislocation method,jejunal tissue was collected to prepare paraffin tissue sections for hematoxylin-eosin staining and histopathological analysis.Enzyme-linked immunosorbent assay was used to determine venous serum inflammatory factors levels including tumor necrosis factor α(TNF-α),interleukin(IL)-1β,IL-6 and high mobility group protein B1(HMGB1).RESULTS:(1)Compared with Con group,the number of colony-forming units was lower in GSNO group and Ag-GSNO NPs group,with statistically significant differences(P<0.05),while the differences in cell proliferative capacity among three groups was not statistically significant(P>0.05).(2)The amounts of NO production were higher in Ag-GSNO NPs group than those in GSNO group at 1 h,2 h,4 h,8 h,12 h and 24 h,with statistically significant differences(P<0.05).(3)On day 0,there were no statistically significant differences in body weights of mice among Control group,Model group,Model+GSNO group and Model+Ag-GSNO NPs group(P>0.05).On the fourth day,compared with Control group,mice in Model group,Model+GSNO group and Model+Ag-GSNO NPs group had lower body weights,lower villus heights,villus widths,crypt depths and crypt numbers in jejunal tissue,higher serum inflammatory factors levels including TNF-α,IL-1β,IL-6 and HMGB1,all with statistically significant differences(P<0.05),mice in Model+Ag-GSNO NPs had higher body weights,villus heights,villus widths,crypt depths and crypt numbers in jejunal tissue,lower serum inflammatory factors levels including TNF-α,IL-1β,IL-6 and HMGB1 than those in Model group and Model+GSNO groups,all with statistically significant differences(P<0.05).CONCLUSIONS:The activity of Ag-GSNO NPs capsules in the treatment of intestinal infections in mouse model with radiation-induced enteritis is superior to that of GSNO powder capsules alone,which can reduced the serum inflammatory factor levels in model mice.关键词
银纳米颗粒/S-亚硝基谷胱甘肽/辐射-诱导肠炎小鼠模型/一氧化氮/抗菌活性Key words
Silver nanoparticles/S-nitrosoglutathione/Model mice with radiation-induced enteritis/Nitric oxide/Antibacterial activity分类
药学引用本文复制引用
卡斯木·卡哈尔,阿依努尔·吐鲁洪,阿迪力·阿不都热合曼..银纳米颗粒-S-亚硝基谷胱甘肽胶囊治疗辐射-诱导肠炎小鼠模型肠道感染的活性研究[J].中国医院用药评价与分析,2025,25(5):539-544,6.基金项目
新疆维吾尔自治区自然科学基金资助项目(No.2023D01C403) （No.2023D01C403）
