中成药2025，Vol.47Issue(5)：1413-1420,8.DOI:10.3969/j.issn.1001-1528.2025.05.001
甘姜苓术汤基准样品量值传递规律研究
Quantitative transmitting rules of benchmark samples of Ganjiang Lingzhu Decoction
摘要
Abstract
AIM To invesigate the quantitative transmitting rules of benchmark samples of Ganjiang Lingzhu Decoction.METHODS HPLC fingerprints were established,after which principal component analysis and partial least squares discriminant analysis were performed.The contents of 6-gingerol,liquiritin and glycyrrhizic acid were determined,after which their transfer rates and dry paste rate were calculated.RESULTS There were 22 common peaks in the fingerprints for 15 batches of benchmark samples with the similarities of more than 0.9.Various batches of benchmark samples were clustered into 3 categories,4 principal components demonstrated the accumulative variance contribution rate of 92.676％,and liquiritin made a great contribution to grouping.In various batches of medicinal materials-decoction pieces,decoction pieces-standard decoctions and standard decoctions-benchmark samples,the transfer rates of 6-gingerol were 84.72％-100.00％,14.29％-24.14％,88.60％-99.36％,those of liquiritin were 74.38％-100.00％,38.54％-64.20％,96.91％-100.30％,and those of glycyrrhizic acid were 68.28％-103.27％,28.48％-49.79％,92.93％-100.49％,the dry paste rate was 13.75％-16.41％.CONCLUSION The preparation process for benchmark samples of Ganjiang Lingzhu Decoction is stable,6-gingerol,liquiritin and glycyrrhizic acid can be taken as their quality markers.Fingerprint combined with content determination can provides a reference for the quality control of Ganjiang Lingzhu Decoction and their related preparations.关键词
甘姜苓术汤/基准样品/量值传递规律/HPLC指纹图谱/含量测定/主成分分析/偏最小二乘法判别分析Key words
Ganjiang Lingzhu Decoction/benchmark samples/quantitative transmitting rules/HPLC fingerprints/content determination/principal component analysis/partial least squares discriminant analysis分类
中医学引用本文复制引用
刘云,李婷,李玲,隋璐,丁越,张彤..甘姜苓术汤基准样品量值传递规律研究[J].中成药,2025,47(5):1413-1420,8.基金项目
上海市科委科技创新项目(19401901800,20S21902500) （19401901800,20S21902500）
上海中医药大学医学攻关产教融合创新平台项目(2023) （2023）
上海市领军人才计划项目(SHLJ2019100) （SHLJ2019100）
