中成药2025，Vol.47Issue(5)：1431-1436,6.DOI:10.3969/j.issn.1001-1528.2025.05.003
UPLC-MS/MS法同时测定加味藿香正气软胶囊中16种成分的含量
Simultaneous content determination of sixteen constituents in Jiawei Huoxiang Zhengqi Soft Capsules by UPLC-MS/MS
摘要
Abstract
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of liquiritin,liquiritin apioside,verbascoside,narirutin,isoacteoside,apigetrin,hesperidin,isoliquiritin,ononin,liquiritigenin,glycyrrhizic acid,isoliquiritigenin,honokiol,obovatol,pogostone and magnolol in Jiawei Huoxiang Zhengqi Soft Capsules.METHODS The analysis was performed on a 40 ℃ thermostatic ZORBAX Eclipse Plus C18 column(2.1 mm×100 mm,1.8 μm),with the mobile phase comprising of 0.1％formic acid-acetonitrile flowing at 0.4 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode.RESULTS Sixteen constituents showed good linear relationships within their own ranges(r＞0.990 0),whose average recoveries were 83.74％-105.12％with the RSDs of 1.10％-4.8％.CONCLUSION This accurate,sensitive,stable and reproducible method can provide a reference for the overall quality control of Jiawei Huoxiang Zhengqi Soft Capsules.关键词
加味藿香正气软胶囊/化学成分/含量测定/UPLC-MS/MSKey words
Jiawei Huoxiang Zhengqi Soft Capsules/chemical constituents/content determination/UPLC-MS/MS分类
药学引用本文复制引用
王倩,高霞,冯健,金彬,陈夏霖,曹亮,李吉峰,张永文,王振中..UPLC-MS/MS法同时测定加味藿香正气软胶囊中16种成分的含量[J].中成药,2025,47(5):1431-1436,6.基金项目
江苏省基础研究计划自然科学基金—前沿引领技术基础研究专项(BK20232014) （BK20232014）
