中成药2025，Vol.47Issue(5)：1449-1455,7.DOI:10.3969/j.issn.1001-1528.2025.05.006
新疆藁本总多酚提取工艺优化、成分分析及其生物活性评价
Extraction process optimization,component analysis and biological activity evaluation for total polyphenols from Conioselinum vaginatu
摘要
Abstract
AIM To optimize the extraction process for total polyphenols from Conioselinum vaginatu(Spreng.)Thell.,make component analysis,and evaluate their anti-oxidant,hypoglycemic activities.METHODS The effects of ultrasound,enzymatic hydrolysis,acid hydrolysis,alcohol extraction and hydrolysis processes on the extraction quantity of total polyphenols were investigated,respectively.With extraction temperature,extraction time,ethanol concetration and liquid-solid ratio as influencing factors,extraction quantity of total polyphenols as an evaluation index,the extraction process was optimized by response surface method.HPLC was adopted in the identification of polyphenolic composition and determination of their contents.Subsequently,total polyphenols' scavenging capacities on DPPH,ABTS,OH free radicals,total reducing power and inhibitory capacity on α-glucosidase were determined.RESULTS The highest extraction quantity of total polyphenols was observable when extraction process was employed.The optimal conditions were determined to be 62 ℃ for extraction temperature,54 min for extraction time,69％for ethanol concentration,and 50∶1 for liquid-solid ratio,the extraction quantity of total polyphenols was(9.51±0.2)mg GAE/g.Seven constituents existed in C.vaginatu,among which ferulic acid demonstrated the highest content,followed by that of myricetin,while D-tryptophan content was the lowest.At the concentration of 7.61 mg/L,total polyphenols displayed the scavenging rates on DPPH,ABTS,OH free radicals of 80.70％,85.97％,28.60％,total reducing power of 0.22,and inhibition rate on α-glucosidase of 77.23％,respectively.CONCLUSION This stable and reliable method can be used for the extraction of total polyphenols from C.vaginatum with strong anti-oxidant,hypoglycemic activities.关键词
新疆藁本/总多酚/提取工艺/成分分析/抗氧化活性/降糖活性/响应面法/HPLCKey words
Conioselinum vaginatu(Spreng.)Thell./total polyphenols/extraction process/component analysis/anti-oxidant activity/hypoglycemic activity/response surface method/HPLC分类
中医学引用本文复制引用
王俊龙,闫慧杰,蔺永刚,李紫薇,时文盼,江升旗,武斌,古庆泽..新疆藁本总多酚提取工艺优化、成分分析及其生物活性评价[J].中成药,2025,47(5):1449-1455,7.基金项目
2023年伊犁州科技计划项目(YJC2023A15) （YJC2023A15）
2024年度伊犁师范大学校级重点项目(2024YSZD009) （2024YSZD009）
