中国农业科学2025，Vol.58Issue(9)：1684-1701,中插1-中插12,30.DOI:10.3864/j.issn.0578-1752.2025.09.002
海岛棉纤维强度QTL紧密连锁InDel分子标记开发及效应评价
Development and Effectiveness Evaluation of InDel Molecular Markers Closely Linked to Fiber Strength QTL in Gossypium barbadense
摘要
Abstract
[Objective]The objective of this study is to develop InDel molecular markers for Island cotton,which is characterized by its superior fiber quality,particularly the fiber tensile strength-a key indicator of cotton fiber quality.The study aims to validate these markers using RIL(Recombinant Inbred Line)populations and resource materials,thereby providing a theoretical foundation for breeding new varieties of Island cotton with enhanced fiber quality.[Method]Utilizing a previously established population of 213 Pima S-7 and 5917 F5:6 RILs,we conducted QTL(Quantitative Trait Locus)mapping to identify the locus regulating fiber strength in Island cotton,designated qFS-chr17-1.InDel markers were designed based on whole genome sequencing(WGS)data of the parental lines,followed by the identification of polymorphic markers.Preliminary validation of these markers was performed using 40 extreme family materials selected based on phenotypic data.Genotyping was carried out on both the 213 RIL population and the 213 Island cotton resource population,alongside multi-year fiber quality data to assess the markers'effectiveness.[Result]The genotyping of the RIL and Island cotton resource populations with the two developed InDel markers indicated a close linkage to fiber strength phenotypic data,with significant differences observed in fiber strength traits among the differentiated materials.The analysis of genotypic combinations revealed an upward trend in fiber strength across four combination types,with materials exhibiting the Hap3(B/A)and Hap4(B/B)genotypes demonstrating significantly greater fiber strength than those with Hap1(A/A)and Hap2(A/B).Furthermore,the InDel-3L2 marker showed significant correlations with fiber length,fiber uniformity,and spinning consistency index,consistent with the observed phenotypic trends.Analysis of multi-year fiber quality data from two experimental sites revealed environmental variability in fiber quality,while temperature data indicated that the developed molecular markers are minimally influenced by environmental factors.Clustering analysis of fiber quality data from 213 Island cotton resource materials,combined with molecular marker genotyping,identified eight materials exhibiting superior fiber quality.[Conclusion]This study successfully developed two InDel molecular markers closely linked to the fiber strength QTL(qFS-chr17-1),which maintain their effectiveness upon combination.The InDel-3L2 marker demonstrates significant correlations with fiber length,fiber uniformity,and spinning consistency index.These markers can efficiently and accurately identify high-strength fiber resources in Island cotton,contributing to the breeding of improved fiber quality.Additionally,eight materials with excellent fiber quality have been identified.关键词
海岛棉/纤维强度/InDel标记/种质资源/组合分子标记Key words
Gossypium barbadense/fiber strength/InDel markers/germplasm resources/combined molecular markers引用本文复制引用
吕涛,孙国清,郭栋材,陈全家,蔡永生,樊标星,曲延英,郑凯..海岛棉纤维强度QTL紧密连锁InDel分子标记开发及效应评价[J].中国农业科学,2025,58(9):1684-1701,中插1-中插12,30.基金项目
国家科技创新2030-重大项目(2023ZD040380103)、国家自然科学基金青年基金(32301867)、新疆维吾尔自治区重点研发项目(2022B02009-1)、"天山英才"培养计划(2023TSYCLJ0012) （2023ZD040380103）
