中国农业科学2025，Vol.58Issue(9)：1702-1718,中插13,18.DOI:10.3864/j.issn.0578-1752.2025.09.003
基于多组学解析谷子后熟米色变化的分子机制
Integrated Multi-Omics Elucidates the Pigmentation Dynamics During Post-Harvest Maturation in Foxtail Millet(Setaria italica)
摘要
Abstract
[Objective]The aim of the study is to investigate the variation characteristics of starch and carotenoid content in grains of the foxtail millet during post-harvest maturation,and to elucidate the molecular mechanism of this phenomenon.The study will expand new insights into carotenoid metabolism in grains,thereby providing theoretical support for the stabilization of millet color,deep processing of millet,and storage.[Method]The carotenoid and starch content in grains from ten varieties of foxtail millet were measured after storage for 0,30,60,and 90 days,respectively.Changes in starch granules of representative materials during the four storage stages were observed using confocal laser scanning microscopy.Transcriptome and proteome analyses were conducted to investigate differential genes and proteins related to starch and carotenoid metabolic pathways at different storage stages for the same material.[Result]Among the 10 foxtail millet varieties,the carotenoid content in the millet mainly showed an upward trend from 0 to 60 days of post-harvest maturation and began to decline after 90 days,but remained higher than that of 0 days.The starch content showed an upward trend after 30 days of post-harvest maturation and then declined overall.Microscopic observation revealed a gradual increase in carotenoid fluorescence in millet starch granules after 30 days of post-harvest maturation,with a tendency for starch plastids to convert into chromoplasts.Furthermore,the transcriptome and proteome analyses identified 1 344 differentially expressed genes(DEGs)and 224 differentially expressed proteins(DEPs),which were mainly enriched in starch degradation and carotenoid synthesis pathways.A metabolic network regulation was also constructed.[Conclusion]During of post-harvest maturation of the foxtail millet,there is an increase trend in the carotenoid content and a decrease trend in the starch content of the millet.It is speculated that starch degradation leads to an increase in pyruvate content,which enters the plastid methylerythritol phosphate(MEP)pathway to promote carotenoid synthesis.Towards the end of post-harvest maturation of the foxtail millet,the downstream synthesis of abscisic acid is inhibited,resulting in the accumulation of substances such as beta-carotene and zeaxanthin,which subsequently affect changes in the millet color.关键词
谷子/后熟/米色/淀粉/类胡萝卜素/转录组学/蛋白质组学Key words
foxtail millet/post-harvest maturation/millet color/starch/carotenoids/transcriptomics/proteomics引用本文复制引用
张义茹,韩雪,姚鑫杰,冯军,魏爱丽,李文超,张彬,韩渊怀,李红英..基于多组学解析谷子后熟米色变化的分子机制[J].中国农业科学,2025,58(9):1702-1718,中插13,18.基金项目
山西省基础研究计划(自由探索类)(20210302124022)、国家自然科学基金面上项目(32070366)、国家自然科学基金联合基金(U21A20216)、山西省高等学校科技创新项目(2020L0533) （自由探索类）
