中成药2025，Vol.47Issue(5)：1467-1476,10.DOI:10.3969/j.issn.1001-1528.2025.05.009
五子衍宗丸对亚急性衰老大鼠睾丸功能损伤及AMPK/mTOR通路的影响
Effects of Wuzi Yanzong Pills on subacute aging-induced testicular trauma and AMPK/mTOR pathway in rats
摘要
Abstract
AIM To investigate the effects of Wuzi Yanzong Pills on subacute aging-induced testicular trauma and AMPK/mTOR pathway in rats.METHODS Rat models of subacute aging were induced by 8-week subcutaneous injection of D-gal(200 mg/kg)into the rat neck,followed by their random assignment into the model group,the metformin group(0.3 g/kg)and the low-dose and high-dose Wuzi Yanzong Pills groups(0.54,2.16 g/kg),with 9 rats in each group,in contrast to the 8 intact rats of the normal group.And the corresponding drug was given by gavage for 4 weeks after modeling,after which the rats had their levels of serum T,FSH,SOD,LH,8-OHdG and MDA detected by ELISA;their pathological changes of testes observed by HE and β-galactosidase staining;and their expressions of aging-related proteins(p16,p21,p53),testicle secretory function-related proteins(CYP11A1,HSD17B3,STAR),autophagy-related proteins(p62,ATG5,Beclin-1,LC3B)and AMPK/mTOR pathway-related protein detected by Western blot and immunohistochemistry.RESULTS Compared with the normal group,the model group displayed morphologically smaller testes and body surface;dry hair;overall atrophic testicular tissue structure;increased testicular protein expressions of γ-H2AX,p16,p21 andβ-galactosidase(P＜0.05,P＜0.01);and decreased p53 protein expression(P＜0.05);suggesting the modeling success.Compared with the model group,the high-dose Wuzi Yanzong Pills group shared decreased levels of serum 8-OHdG,MDA,FSH and LH(P＜0.01);increased levels of SOD and T(P＜0.01);improved sperm damage and almost morphologically normal testicular tissue;decreased testicular protein expressions of p16,p21,p53,p62 and p-mTOR(P＜0.01);and increased protein expressions of CYP11A1,HSD17B3,STAR,ATG5,Beclin-1,LC3B and p-AMPK(P＜0.01).CONCLUSION Wuzi Yanzong Pills can improve the subacute aging-induced testicular injury and testicular function in rats by reducing their oxidative stress through improving their autophagy level and testicle antioxidant capacity.关键词
五子衍宗丸/亚急性衰老大鼠/睾丸功能损伤/氧化应激/自噬/AMPK/mTOR通路Key words
Wuzi Yanzong Pills/subacute aging rats/testicular function damage/oxidative stress/autophagy/AMPK/mTOR pathway分类
医药卫生引用本文复制引用
穆胜,马会明,惠宏,蒲静,苏骁..五子衍宗丸对亚急性衰老大鼠睾丸功能损伤及AMPK/mTOR通路的影响[J].中成药,2025,47(5):1467-1476,10.基金项目
国家自然科学基金(82274624) （82274624）
宁夏自然科学基金(2023AAC03211) （2023AAC03211）
