中国农业科学2025，Vol.58Issue(9)：1767-1778,12.DOI:10.3864/j.issn.0578-1752.2025.09.007
不同抗性葡萄响应白腐病侵染的次生代谢物变化
Changes of Secondary Metabolites in Grapes with Different Resistance Levels in Response to White Rot Infection
摘要
Abstract
[Objective]This study aims to explore the role of secondary metabolites in grape resistance to white rot,and to identify the metabolites associated with grape resistance to white rot.[Method]The fruits of disease resistant Vitis davidii 0941(Vd)and the disease susceptible Vitis vinifera Manicure Finger(Vv)at color transition stage were used as experimental materials.The fruit pedicel was pricked to create a wound and inoculated with the white rot pathogen.Fruits were collected at different time points(0,24,48 h)after the removal of infected parts following pathogen inoculation,and a broad-targeted metabolomics approach was employed to analyze the metabolites in the resistant and susceptible varieties.[Result]A total of 960 metabolites were detected in the metabolome,which were divided into 12 major categories,such as amino acids and their derivatives,phenolic acids,nucleotides and their derivatives,flavonoids,and lipids.Principal component analysis(PCA)and hierarchical cluster analysis(HCA)showed significant differences in metabolites between the resistant variety Vd and the susceptible variety Vv before and after infection with white rot.Using|log2 fold change|≥1 and P-value≤0.01 as the threshold for screening differential metabolites,a total of 501 differential metabolites were identified.After infection with white rot,Vd and Vv exhibited different metabolic responses,especially at 24 and 48 hours post-infection,where the number and magnitude of changes in differential metabolites were more significant in the susceptible variety.KEGG enrichment analysis showed that these differential metabolites were mainly enriched in the metabolic pathways of biosynthesis of secondary metabolites,flavone and flavonol biosynthesis,ABC transporters,ascorbate and aldarate metabolism,and biosynthesis of amino acids.WGCNA identified metabolites significantly related to disease resistance,obtaining 10 secondary metabolites that may be related to disease resistance,including one amino acid and its derivative(O-acetylserine),one phenolic acid(arbutin),one flavonoid(cyanidin-3-O-(6''-O-caffeoyl)glucoside),and seven terpenoids(α-amyrenone,botulin,3-epiursolic acid,2-hydroxyoleanolic acid,maslinic acid,alphitolic acid,3,24-dihydroxy-17,21-semiacetal-12(13)oleanolic acid).[Conclusion]This study reveals the changes in grape metabolites under white rot infection,and these secondary metabolites(amino acids and their derivatives,phenolic acids,flavonoids,and terpenoids)that are up regulated in the resistant variety V.davidi 0941 may play a significant role in the resistance to white rot.关键词
葡萄/白腐病/抗病/代谢组学/次生代谢物Key words
grape/white rot/disease resistance/metabolomics/secondary metabolite引用本文复制引用
谭西北,兰徐颖,刘崇怀,樊秀彩,姜建福,孙磊,李鹏,余书鑫,张颖..不同抗性葡萄响应白腐病侵染的次生代谢物变化[J].中国农业科学,2025,58(9):1767-1778,12.基金项目
国家重点研发计划(2021YFD1200202)、中国农业科学院科技创新工程(CAAS-ASTIP-2016-ZFRI-02)、国家现代农业产业技术体系建设专项资金(CARS-29-yz-1) （2021YFD1200202）
