中国中医药信息杂志2025，Vol.32Issue(6)：80-85,6.DOI:10.19879/j.cnki.1005-5304.202409610
重楼皂苷Ⅲ对结肠癌细胞HCT116增殖、周期、凋亡的影响及其作用机制
Effects and Mechanism of Polyphyllin Ⅲ on Proliferation,Cycle and Apoptosis of Colon Cancer Cell HCT116
摘要
Abstract
Objective To investigate the effects of polyphyllin Ⅲ on proliferation,cycle and apoptosis of colon cancer cells HCT116;To explore its mechanism in treating colon cancer.Methods Colon cancer cells HCT116 were cultured in vitro,the cells were dived into control group and polyphyllin Ⅲ low-,medium-and high-dosage group,drug-free media and low-,medium-and high-concentration of polyphyllin Ⅲ were applied to the cells.Cell proliferation was detected by CCK-8 method,and cell cycle and apoptosis were detected by flow cytometry,the expressions of cyclinD1,CDK4,CDK6,RB,p-RB,Bax,Bcl-2,Bcl-xl mRNAs and proteins were detected by RT-PCR and Western blot.Results Compared with the control group,the HCT116 cell viability decreased in the polyphyllin Ⅲ groups with different dosages(P<0.01),the proportion of G0-G1 phase cells increased(P<0.01),the proportion of S,G2-M phase cells decreased(P<0.01),the cell apoptosis rate increased(P<0.01),and the expressions of cyclinD1,CDK4,CDK6,Bcl-2,Bcl-xl mRNA and cyclinD1,CDK4,CDK6,p-RB,Bcl-2,Bcl-xl proteins decreased,while the expression of Bax mRNA and protein increased,with a dosage-dependent manner(P<0.05,P<0.01).Conclusion Polyphyllin Ⅲ can inhibit the proliferation of colon cancer cells cultured in vitro and promote cell apoptosis,blocking cells in the G0-G1 phase.The mechanism may be related to the inhibition of cell cycle related proteins and apoptosis related protein expression.关键词
重楼皂苷Ⅲ/结肠癌/增殖/细胞周期/凋亡/HCT116细胞Key words
polyphyllin Ⅲ/colon cancer/proliferation/cell cycle/apoptosis/HCT116 cells分类
医药卫生引用本文复制引用
罗燕,蒋益兰,曾千,罗玲,张轩,罗吉..重楼皂苷Ⅲ对结肠癌细胞HCT116增殖、周期、凋亡的影响及其作用机制[J].中国中医药信息杂志,2025,32(6):80-85,6.基金项目
国家自然科学基金青年科学基金(82004312) （82004312）
湖南省自然科学基金面上项目(2021JJ30421) （2021JJ30421）
湖南省自然科学基金青年基金(2021JJ40312) （2021JJ40312）
湖南省临床医疗技术创新引导项目(2021SK51006) （2021SK51006）
湖南省教育厅优秀青年项目(20B443) （20B443）
长沙市自然科学基金(kq2014204) （kq2014204）
湖南省中医药管理局重点项目(A2024018) （A2024018）
