中国中医急症2025，Vol.34Issue(5)：787-790,805,5.DOI:10.3969/j.issn.1004-745X.2025.05.008
运脾开胃方调控Ghrelin/GHSR/MLCK改善脓毒症大鼠肠黏膜屏障功能的研究
Mechanism of Yunpi Kaiwei Decoction in Regulating Ghrelin/GHSR/MLCK to Improve Intestinal Mucosal Barrier Function in Sepsis Rats
摘要
Abstract
Objective:To evaluate the intervention effect of Yunpi Kaiwei Decoction on intestinal mucosal barri-er injury in a sepsis animal model and to explore its mechanism.Methods:60 SD rats were divided into four groups:a sham operation group,a model group,a Yunpi Kaiwei Decoction group,and a Yunpi Kaiwei Decoction+Ghrelin receptor(GHSR)antagonist group.Serum levels of ghrelin,tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and interleukin-6(IL-6)were measured using enzyme-linked immunosorbent assay(ELISA).Histo-pathological changes in gastrointestinal tissues were observed using hematoxylin-eosin(HE)staining,and intesti-nal epithelial structure was examined by transmission electron microscopy.Intestinal permeability was assessed us-ing the Evans blue method.Protein expression levels of Ghrelin,GHSR,myosin light chain kinase 1(MLCK1),phosphorylated MLCK1(p-MLCK1),myosin light chain(MLC),and phosphorylated MLC(p-MLC)in colon tissue were detected by Western blotting.Results:Compared with the model group,the Yunpi Kaiwei Decoction group showed increased serum ghrelin levels(P＜0.01)and decreased pro-inflammatory cytokines TNF-α,IL-1β,and IL-6(P＜0.01).After GHSR antagonism,pro-inflammatory cytokine levels increased(P＜0.05 or P＜0.01).Com-pared with the model group,the Yunpi Kaiwei Decoction group exhibited reduced gastrointestinal pathological changes and decreased intestinal permeability(P＜0.01).It also upregulated the expression of Ghrelin and GHSR in colon tissue(P＜0.01).However,after GHSR antagonism,the pathological effects were weakened,and intestinal permeability increased(P＜0.05).The p-MLCK/MLCK and p-MLC/MLC ratios decreased after Yunpi Kaiwei De-coction intervention,while these ratios increased after GHSR antagonism.Conclusion:Yunpi Kaiwei Decoction can improve intestinal mucosal barrier function in sepsis,and its mechanism may be mediated by the Ghrelin/GH-SR/MLCK signaling pathway.关键词
脓毒症/肠黏膜屏障/运脾开胃方/Ghrelin/大鼠Key words
Sepsis/Intestinal mucosal barrier/Yunpi Kaiwei Decoction/Ghrelin/Rats分类
医药卫生引用本文复制引用
胡云霞,郑晨霞,赵智强,白宇,高慧,陈明祺,张海东,吕天璞,王建林,吴涛,李童童..运脾开胃方调控Ghrelin/GHSR/MLCK改善脓毒症大鼠肠黏膜屏障功能的研究[J].中国中医急症,2025,34(5):787-790,805,5.基金项目
江苏省中医药科技发展计划(MS2023092) （MS2023092）
常州市科技计划资助项目(CJ20239006) （CJ20239006）
常州市卫生健康委员会青年人才科技项目(QN202137) （QN202137）
2023年度常州市"龙城医星"卫生青年科技人才托举工程(lcyx2023004) （lcyx2023004）
溧阳市青年科技人才托举工程(溧科协[2023]19号) （溧科协[2023]19号）
江苏省中医院溧阳分院科技项目(YN202105) （YN202105）
