中国茶叶2025，Vol.47Issue(5)：59-65,7.
7份大叶种茶树种质资源子叶再生体系的比较研究
Comparative Study on Cotyledon Regeneration System of 7 Germplasm Resources of Camellia sinensis var.assamica
摘要
Abstract
To establish the somatic embryo regeneration system of Camellia sinensis var.assamica,the cotyledons were used as explants to explore the conditions of explant disinfection,somatic embryo induction and somatic em-bryo seedling formation of 7 resources of Camellia sinensis var.assamica from Yunnan.The results show that the contamination rate was zero under the condition of soaking in 75％ethanol for 1 min and then disinfecting with 0.1％HgCl2 for 16 min.The induction rates of somatic embryos ranged from 0 to 50.84％among 7 resources.The induction rate of 'Linxiang 28' was the highest(50.84％),followed by 'Xiangguiyinhao'(40.09％).Compared with the control group,the addition of 6-BA and 2,4-D significantly increased the induction rate of somatic embryos.The somatic embryo seedling rates of 'Linxiang 28' and 'Xiangguiyinhao' were 21.21％and 1.85％,respectively.This study established a complete somatic embryogenesis regeneration system of Camellia sinensis var.assamica by using 'Xiangguiyinhao' and 'Linxiang 28',which laid a foundation for the preservation of germplasm resources and the construction of genetic transformation system.关键词
茶/子叶/体细胞胚/再生体系Key words
Camellia sinensis L/cotyledons/somatic embryo/regeneration system分类
农业科技引用本文复制引用
储晓艳,吴丽,杨慧琳,王绍梅,刘艳红,李晓君,郭飞..7份大叶种茶树种质资源子叶再生体系的比较研究[J].中国茶叶,2025,47(5):59-65,7.基金项目
云南省科技厅重点实验室建设项目(202449CE340010)、临沧市建设国家可持续发展议程创新示范区科技专项(202204AC100001-A01) （202449CE340010）
