中国中医药信息杂志2025，Vol.32Issue(6)：86-92,7.DOI:10.19879/j.cnki.1005-5304.202409130
参附注射液抑制铁死亡对大鼠心肌细胞氧化应激损伤的影响
Effects of Shenfu Injection in Inhibiting Ferroptosis on Oxidative Stress Injury in Rat Cardiomyocytes
摘要
Abstract
Objective To observe the effects of Shenfu Injection on ferroptosis-related factors and oxidative stress-related indexes in rat cardiomyocytes treated with isoproterenol;To explore its mechanism in treating chronic heart failure.Methods Isoproterenol was used to induce rat H9c2 cardiomyocyte injury.The cells were divided into normal group,model group,Shenfu Injection group and Ferrostatin-1 group,and treated with corresponding intervention.Transmission electron microscopy was used to observe the ultrastructure of cellular mitochondria,ferrous ion fluorescent probe was used to detect Fe2+content in cells,flow cytometry was used to detect intracellular contents of ROS and Lipid-ROS;colorimetry was used to detect the contents of MDA,GSH,SOD and GSH-Px in cells;Western blot and RT-qPCR were used to detect the protein and mRNA expressions of GPX4,FTH1,SLC7A11,p53,COX2 and Nrf2 in cells.Results Compared with the normal group,the survival rate of H9c2 cells in the model group was significantly reduced(P<0.01),with cell swelling and rupture,mitochondrial shrinkage,decreased quantity and disordered arrangement,more damaged mitochondria,the contents of Fe2+,ROS,Lipid-ROS and MDA in cells significantly increased(P<0.01),while the contents of GSH,SOD and GSH-Px significantly decreased(P<0.01),the expressions of p53,COX2 protein and mRNA significantly increased(P<0.01),the expressions of GPX4,FTH1,SLC7A11 and Nrf2 protein and mRNA significantly decreased(P<0.01).Compared with the model group,the survival rates of H9c2 cells in Shenfu Injection group and Ferrostatin-1 group significantly increased(P<0.01),there was a larger number of normal mitochondria and a more complete structure,the contents of Fe2+,ROS,Lipid-ROS and MDA in cells were significantly decreased(P<0.01),while the contents of GSH,SOD and GSH-Px were significantly increased(P<0.05,P<0.01),the expressions of p53,COX2 protein and mRNA were significantly decreased(P<0.05,P<0.01),while the expressions of GPX4,FTH1,SLC7A11 and Nrf2 protein and mRNA significantly increased(P<0.01).Conclusion Shenfu Injection can reduce p53 expression,weaken its inhibitory effect on SLC7A11,thereby promoting GPX4 expression,inhibiting ferroptosis,reducing lipid peroxide accumulation,increasing cellular antioxidant capacity,and alleviating myocardial cell oxidative damage.关键词
慢性心力衰竭/参附注射液/铁死亡/氧化应激/H9c2细胞Key words
chronic heart failure/Shenfu Injection/ferroptosis/oxidative stress/H9c2 cells分类
医药卫生引用本文复制引用
王梓仪,郭瑾,张倩,廖晓倩,范星宇,胡志希,何庆勇..参附注射液抑制铁死亡对大鼠心肌细胞氧化应激损伤的影响[J].中国中医药信息杂志,2025,32(6):86-92,7.基金项目
国家自然科学基金(82274412) （82274412）
中央高水平中医医院临床研究和成果转化能力提升项目(HLCMHPP2023064) （HLCMHPP2023064）
首都临床特色诊疗技术研究及转化应用项目(Z221100007422081) （Z221100007422081）
