中国中医药信息杂志2025，Vol.32Issue(6)：99-104,6.DOI:10.19879/j.cnki.1005-5304.202409609
基于GSN、PSAP及NF-κB通路探讨活血通降方对反流性食管炎模型大鼠食管黏膜损伤的影响
Exploration on the Effects of Huoxue Tongjiang Decoction on Esophageal Mucosa Injury in Reflux Esophagitis Model Rats Based on the GSN,PSAP and NF-κB Pathway
摘要
Abstract
Objective To observe the effects of Huoxue Tongjiang Decoction on the expressions of gelsolin(GSN),prosaposin(PSAP)and nuclear factor(NF)-κB in reflux esophagitis(RE)model rats;To explore its mechanism of protecting esophageal mucosa.Methods Totally 75 Wistar rats were randomly divided into control group,model group,Western medicine group,TCM low-and high-dosage groups,with 15 rats in each group.The control group was given celiac switch operation,the other groups were given modified partial cardiac myotomy and external pyloric partial ligation to prepare RE rat model.Seven days after operation,each group was given normal saline,Western medicine or corresponding dosage of Huoxue Tongjiang Decoction for gavage,twice a day for 14 days.HE staining was used to observe the morphology of esophageal mucosa and pathological score was evaluated,immunohistochemical staining was used to detect the positive expressions of GSN and PSAP in esophageal mucosa,and Western blot was used to detect the protein expressions of NF-κBp65 and p-NF-κBp65 in esophageal tissue,ELISA was used to detect the contents of serum tumor necrosis factor(TNF)-α and interleukin(IL)-6.Results Compared with the control group,the esophageal mucosa of the model group was obviously damaged,the pathological score increased,the expression of GSN in esophageal mucosa decreased,the expressions of PSAP,NF-κBp65 and p-NF-κBp65 protein increased,and the contents of TNF-α and IL-6 in serum increased,with statistical significance(P<0.01).Compared with the model group,the esophageal mucosal injury improved obviously in the Western medicine group and TCM low-and high-dosage groups,the pathological score decreased,the expression of GSN in esophageal mucosa increased,the expressions of PSAP,NF-κBp65 and p-NF-κBp65 protein decreased,and the contents of TNF-α and IL-6 in serum decreased,with statistical significance(P<0.05,P<0.01).Conclusion Huoxue Tongjiang Decoction can effectively improve the immune inflammation of esophageal mucosa in RE model rats,its mechanism may be related to regulating GSN,PSAP and NF-κB pathway and reducing the release of inflammatory factors TNF-α and IL-6.关键词
反流性食管炎/活血通降方/核因子-κB/凝溶胶蛋白/鞘脂激活蛋白原/大鼠Key words
reflux esophagitis/Huoxue Tongjiang Decoction/NF-κB/GSN/PSAP/rats分类
医药卫生引用本文复制引用
刘思雨,唐艳萍,刘磊,李培彩,杨磊..基于GSN、PSAP及NF-κB通路探讨活血通降方对反流性食管炎模型大鼠食管黏膜损伤的影响[J].中国中医药信息杂志,2025,32(6):99-104,6.基金项目
国家自然科学基金面上项目(82274253、82074213) （82274253、82074213）
国家自然科学基金青年科学基金(82204988) （82204988）
