中成药2025，Vol.47Issue(5)：1531-1539,9.DOI:10.3969/j.issn.1001-1528.2025.05.018
皖产葛根化学成分及其体外降脂活性研究
Chemical constituents from Radix Puerariae Lobatae from Anhui province and their in vitro lipid-lowering activities
摘要
Abstract
AIM To study the chemical constituents from Radix Puerariae Lobatae from Anhui province and their in vitro lipid-lowering activity.METHODS D101 macroporous resin,silica gel,MCI,and semi-preparative HPLC were used for isolation and purification,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The lipid accumulation model was established by palmitic acid-induced hepatocyte AML12,and the in vitro lipid-lowering activity was evaluated.RESULTS Twenty-one compounds were isolated and identified as carboxymethyl isoferulate(1),methyl(E)feruloylglycolate(2),ariscucurbin-A(3),trans-p-coumaroygly colic acid(4),indole-3-carboxaldehyde(5),β-sitosterol(6),puerol A(7),pueroside C(8),cumoestrol(9),dihydrofurocoumarin(10),decuroside V(11),psoralen dimer(12),bergapten dimer(13),isoliquiritigenin(14),neoliquiritin(15),calycosin(16),daidzein(17),3,4,7-trihydroxyisoflavone(18),ononin(19),genistein(20),genistin(21).Compounds 1-4、7-10、13 and 17-19 significantly inhibited lipid accumulation in AML12 cells,and compound 9 inhibited PPAR-γ expression.CONCLUSION Compounds 1-6,10-13 are isolated from Pueraria genus for the first time.Compounds 1-4,7-10,13 and 17-19 have good lipid-lowering activity.关键词
葛根/乙酸乙酯部位/化学成分/分离鉴定/体外降脂活性Key words
Radix Puerariae Lobatae/ethyl acetate fraction/chemical constituents/isolation and identification/in vitro lipid-lowering activity分类
医药卫生引用本文复制引用
喻杰,杨著,吕秋月,王国栋,韩军,吴江平..皖产葛根化学成分及其体外降脂活性研究[J].中成药,2025,47(5):1531-1539,9.基金项目
安徽省高等学校自然科学基金(2024AH040169,2024AH051923,2024AH051934) （2024AH040169,2024AH051923,2024AH051934）
合肥综合性国家科学中心大健康研究院揭榜挂帅项目(2023CXMMTCM011) （2023CXMMTCM011）
安徽中医药大学国家中医药管理局高水平中医药重点学科中药化学学科开放课题(HKDCCM2024008) （HKDCCM2024008）
