中国中医药信息杂志2025，Vol.32Issue(6)：142-146,5.DOI:10.19879/j.cnki.1005-5304.202502180
基于DNA条形码ITS序列的杜鹃兰与山慈菇其他基原药材及混伪品分子鉴定
Molecular Identification of Cremastra appendiculata and Other Original Medicinal Materials of Cremastrae Pseudobulbus and Confusing Products Based on ITS Sequences in DNA Barcodes
摘要
Abstract
Objective To distinguish Cremastra appendiculata(D.Don)Makino,Pleione yunnanensis Rolfe and Pleione bulbocodioides,and its easily confusing products Oreorchis patens and Iphigenia indica Kunth using the ITS sequence in DNA barcodes;To explore the genetic diversity of Cremastra appendiculata germplasm resources.Methods Three different original Cremastra appendiculata,Pleione yunnanensis Rolfe and Pleione bulbocodioides,and their easily confusing products Cremastrae Pseudobulbus of Oreorchis patens and Iphigenia indica Kunth were selected as the research objects,and the genomic DNA of the above samples were extracted by the modified CTAB method,and then the ITS sequences were amplified,sequenced and spliced by PCR technology.The Kimura 2-Parameter(K2P)model was used to calculate the genetic distance,and the phylogenetic tree was constructed with the help of neighbour joining method(NJ)for genetic relationship analysis.Results Except for the Iphigenia indica Kunth species that were not found during the BLAST search,the BLAST comparison results of the other samples were higher than 95%.At the same time,the results of phylogenetic tree showed that Cremastra appendiculata,Pleione yunnanensis Rolfe and Pleione bulbocodioides were clustered into one branch,respectively,and the easily confusing products were also respectively clustered into one branch.Conclusion The ITS sequence in DNA barcodes can be used to accurately distinguish Cremastra appendiculata,Pleione yunnanensis Rolfe and Pleione bulbocodioides,and its easily confusing products Oreorchis patens and Iphigenia indica Kunth.关键词
杜鹃兰/山慈菇/ITS序列/DNA条形码/分子鉴定Key words
Cremastra appendiculata/Cremastrae Pseudobulbus/ITS sequence/DNA barcodes/molecular identification分类
医药卫生引用本文复制引用
张金玲,李月婷,肖秋肖,王倩,周思佳,龙亚丽,黄勇,郑林,巩仔鹏,金阳..基于DNA条形码ITS序列的杜鹃兰与山慈菇其他基原药材及混伪品分子鉴定[J].中国中医药信息杂志,2025,32(6):142-146,5.基金项目
国家自然科学基金(82360765) （82360765）
贵州省科技计划项目(黔科合平台人才-CXTD[2023]019) （黔科合平台人才-CXTD[2023]019）
贵州省高层次创新型人才项目(黔科合平台人才-GCC[2023]036、黔科合平台人才-GCC[2022]031-1) （黔科合平台人才-GCC[2023]036、黔科合平台人才-GCC[2022]031-1）
