浙江中医药大学学报2025，Vol.49Issue(4)：391-400,10.DOI:10.16466/j.issn1005-5509.2025.04.001
益气补肾方调控环状RNA circ_0051246抗胃癌干细胞增殖和迁移的机制研究
Study on the Mechanism of Yiqi Bushen Formula Regulating Circular RNA Circ_0051246 Against Proliferation and Migration of Gastric Cancer Stem Cells
摘要
Abstract
[Objective]To investigate the mechanism action of Yiqi Bushen Formula on the proliferation and migration of gastric cancer stem cells(GCSCs).[Methods]SGC-7901 GCSCs with double positive markers of CD44 and epithelial cell adhesion molecule(EpCAM)were sorted by immunomagnetic beads.The selected CD44+EpCAM+SGC-7901 GCSCs were subcultured and stably transfected with circ_0051246 control,pcDNA circ_0051246 and si-circ_0051246 respectively.The above four groups of cells(including untransfected)were divided into three subgroups:blank control group,negative control group and Yiqi Bushen Formula group.The blank control group was normally subcultured,the negative control group was intervened with blank serum,and the Yiqi Bushen Formula group was intervened with Yiqi Bushen Formula-containing serum.The microstructure of SGC-7901 GCSCs was observed by laser confocal and transmission electron microscopy.Cell counting kit-8(CCK-8)and cell cloning experiments were used to detect the proliferation ability of SGC-7901 GCSCs.Flow cytometry was used to detect the positive rate of CD44 and EpCAM and the apoptosis rate.Cell scratch test and transwell were used to detect the migration and invasion ability of SGC-7901 GCSCs.The expression of circ_0051246 in each experimental group was detected by quantitative reverse transcription-polymerase chain reaction(qRT-PCR).[Results]Compared with respective negative control group,the number of mitochondrial cristae in each group of Yiqi Bushen Formula was significantly reduced,the endoplasmic reticulum was significantly swollen.Compared with respective blank control group,the expression of actin in the cytoskeleton structure of each group of Yiqi Bushen Formula was significantly decreased.Compared with respective negative control group,the cell proliferation ability of Yiqi Bushen Formula groups was weakened(P＜0.05,P＜0.01),the rate of double positive cells of CD44 and EpCAM antibodies was significantly decreased(P＜0.01),the cloning ability was significantly decreased(P＜0.01),the apoptosis rate was significantly increased(P＜0.01),the migration rate was significantly decreased at 24 and 48 h(P＜0.05,P＜0.01),the number of cell migration and invasion was significantly reduced(P＜0.01).The expression level of circ_0051246 in Yiqi Bushen Formula group was significantly decreased(P＜0.01).[Conclusion]Yiqi Bushen Formula may play a role in anti-proliferation and migration of GCSCs by inhibiting the expression of circ_0051246 and reducing the expression of SGC-7901 CSCs surface markers CD44 and EpCAM,which provides a new idea for the treatment of gastric cancer.关键词
胃癌/肿瘤干细胞/增殖/迁移/circ_0051246/细胞转染/益气补肾方/机制研究Key words
gastric cancer/cancer stem cells/proliferation/migration/circ_0051246/cell transfection/Yiqi Bushen Formula/mechanism research分类
医药卫生引用本文复制引用
徐叶峰,邓明慧,姚勇伟,严卿莹,程淼,刘云霞..益气补肾方调控环状RNA circ_0051246抗胃癌干细胞增殖和迁移的机制研究[J].浙江中医药大学学报,2025,49(4):391-400,10.基金项目
国家自然科学基金面上项目(82074209) （82074209）
浙江省名老中医专家传承工作室建设项目(GZS2020030)General Program of National Natural Science Foundation Project(82074209) （GZS2020030）
Zhejiang Province Famous Traditional Chinese Medicine Experts Inheritance Studio Construction Project(GZS2020030) （GZS2020030）
