种子2025，Vol.44Issue(4)：43-53,11.DOI:10.16590/j.cnki.1001-4705.2025.04.043
基于转录组的北乌头、芍药种子休眠NAC家族成员鉴定与分析
Identification and Analysis of Dormant NAC Family Members of Aconitum kusnezoffii and Paeonia lactiflora Seeds Based on Transcriptome
摘要
Abstract
The NAC gene families based on transcriptome data of Aconitum kusnezoffii and Paeonia lactiflora seeds under low-temperature stratification were identified by using bioinformatics methods.A total of 33 AKNAC and 37 PLNAC family genes were identified.The results showed that the number of amino acids in AKNAC and PLNAC was 85 to 661 and 80 to 654,respectively.The molecular weights were 10.13 to 67.69 kD and 9.38 to 73.87 kD respectively.The isoelectric points were 4.74 to 9.84 and 4.78 to 10.05 respectively.The instability coefficients were 23.13 to 57.46 and 22.91 to 57.53 respectively.The lipid solubility indices were 53.96 to 90.14 and 30.05 to 82.00 respectively.The hydrophilic coefficients were-0.909 to 0.014 and-1.245 to-0.072 respectively,all belonging to hydrophilic proteins.The Most proteins were located in the cell nucleus,and there were certain similarities among the members of the NAC family.The results of the evolutionary tree showed that there were certain similarities among the Motif,Domain,and NAC sequence structures.The relative expression levels and changing trends at different stages of different NAC family members in Aconitum kusnezoffii and Paeonia lactiflora during stratification were different.The expression levels of some NAC family members increased during low-temperature periods,reflecting the characteristics of NAC responding to abiotic stress.And some family members with similar trends in expression levels were clustered together,reflecting the adaptability between structure and function.关键词
NAC基因家族/北乌头/芍药/生物信息学分析Key words
NAC gene family/Aconitum kusnezoffii/Paeonia lactiflora/bioinformatics analysis分类
农业科技引用本文复制引用
乌兰图雅,路景诗,贾俊英,侯伟峰,潮乐蒙,张晓明..基于转录组的北乌头、芍药种子休眠NAC家族成员鉴定与分析[J].种子,2025,44(4):43-53,11.基金项目
内蒙古自治区自然科学基金项目(2024LHMS03047) （2024LHMS03047）
内蒙古自治区高校中(蒙)药材生态种植工程研究中心(培育)项目 （蒙）
河套灌区生态环境治理与修复技术创新团队(HTKCT-A202408) （HTKCT-A202408）
