种子2025，Vol.44Issue(4)：70-77,8.DOI:10.16590/j.cnki.1001-4705.2025.04.070
基于转录组测序的多伞阿魏FfeFLS基因克隆与分析
Cloning and Analysis of FfeFLS Gene from Ferula feruloides Based on Transcriptome Sequencing
摘要
Abstract
The flavonol synthase gene(FLS)has the function of significantly increasing the flavonoid content in plants.The FfeFLS gene was obtained through gene cloning from Ferula feruloides,and the protein encoded by it was predicted and analyzed.The results showed that the isoelectric point of the protein was 5.67,which was a hydrophilic protein,and there were many functional elements relat-ed to flavonoid synthesis,hormones,and light responses.The gene sequence had the highest homolo-gy with carrots.The results of qRT-PCR indicated that FfeFLS multiferulic was expressed in April,May and June,but the expression level in June was significantly higher than that in other months.关键词
多伞阿魏/FfeFLS基因/生物信息学分析/基因克隆Key words
Ferula feruloides/FfeFLS gene/bioinformatics analysis/gene cloning分类
生物科学引用本文复制引用
赵静怡,郭新勇,赵金提,刘芯宇,田志佳,余香雪,李倩,陈博,木古丽,张定国..基于转录组测序的多伞阿魏FfeFLS基因克隆与分析[J].种子,2025,44(4):70-77,8.基金项目
国家自然科学基金地区基金项目(32160071) （32160071）
伊犁哈萨克自治州科技计划项目(YYD2023A08) （YYD2023A08）
