作物杂志Issue(3)：52-60,9.DOI:10.16035/j.issn.1001-7283.2025.03.008
高淀粉马铃薯初级核心种质资源的遗传多样性分析
Genetic Diversity Analysis of High Starch Potato Primary Core Germplasm Resources
摘要
Abstract
A total of 62 high-starch potato primary core germplasm resources from 13 countries and organizations was used as experimental materials to analyze the genetic diversity of the high-starch potato primary core germplasms using 54 phenotypic trait data and SSR molecular data of 19 pairs of polymorphic primers.The results showed that the 62 primary core germplasm resources contained most of descriptors of the 54 phenotypic traits of the potato description specification,only eight traits did not contain whole descriptors,and the missing descriptors are mostly the types of lacking and scarce resources in Chinaʼs resource pool.Diversity index for the 47 quality traits ranged from 0.0633 to 1.3043,and the average diversity index was 0.8197.Diversity indexes of the seven quantitative traits were between 1.0701 and 1.3152,and the average genetic diversity index was 1.1630.The genetic diversity of quantitative traits was generally higher than that of quality traits,with more variation,which was conducive to the selection of germplasm resources in practical application.By performing the correlation analysis of the seven quantitative traits,the correlation between the starch content and other quantitative traits were clarified.The 19 pairs of primers amplified 116 polymorphic bands,and the percentage of polymorphism was 96.72%.On average,each pair of primers amplified 6.26 bands,with an average of 6.26 alleles and 3.9931 effective alleles.Neiʼs gene diversity index varied from 0.3735 to 0.8480,and the Shannon-Wiener diversity index varied from 0.6624 to 2.0123.Cluster analysis was conducted using 54 phenotypic data and 19 pairs of polymorphic primer SSR molecular data,both of which were able to classify 62 high-starch potato primary core germplasms into four groups.The classification results based on the two data have an overlap degree of about 50%,reflecting the genetic diversity and genetic relationship of 62 germplasm resources at the two levels of phenotype and gene.关键词
马铃薯/核心种质/遗传多样性/分子数据Key words
Potato/Core germplasm/Genetic diversity/Molecular data引用本文复制引用
孙邦升,宋继玲,杨梦平,邢金月,胡尊艳,郝智勇,李菁华,刘春生..高淀粉马铃薯初级核心种质资源的遗传多样性分析[J].作物杂志,2025,(3):52-60,9.基金项目
国家马铃薯种质资源安全保存项目(19230823) （19230823）
黑龙江省农业创新跨越工程(CX23TS24) （CX23TS24）
黑龙江省农业科学院院级课题(2020YYYF003) （2020YYYF003）
