中山大学学报（自然科学版）（中英文）2025，Vol.64Issue(3)：60-73,14.DOI:10.13471/j.cnki.acta.snus.ZR20240347
基于双发射碳量子点的比率型荧光探针在Mn2+传感与细胞成像中的应用
A dual-emission carbon dots-based ratiometric sensor for detection and cellular imaging of Mn2+ions
摘要
Abstract
Manganese(Mn),an essential trace element in the human body,plays critical roles in many biological processes.Recent studies have discovered that Mn2+may promote or directly activate the cGAS-STING pathway,thereby subsequently initiating the natural immune response and augmenting antitumor therapy.However,the current lack of accurate methods for Mn2+determination in cells significantly limits their mechanism investigation;hence,it is urgent to establish novel tools to detect Mn2+in cells.In this study,the dual-emission carbon dots were initially synthesized via the one-pot hydrothermal method employing L-aspartic acid and p-phenylenediamine as raw materials.In the presence of Mn2+,the emission peak centered at 350 nm exhibited significant enhancement,whereas another peak at 610 nm remained stable.Consequently,a ratiometric sensor for Mn2+determination was established using the signal at 350 nm as the responsive signal and the signal at 610 nm as an internal reference.Under the optimal condition,a good linear relationship was achieved between the F350/F610 value and Mn2+concentration ranging from 0.9 to 15 μ mol/L,with a calculated LOD of 61 nmol/L.Benefiting from the special Mn2+-induced ratiometric approach,this method demonstrates outstanding sensitivity,selectivity,and stability,rendering it applicable for Mn2+determination in complex biological samples,as well as Mn2+imaging in MKN-45 and LO2 cells.关键词
Mn2+/碳量子点/比率型/细胞成像/荧光Key words
Mn2+/carbon dots/ratiometric/cell imaging/fluorescence分类
化学引用本文复制引用
张越诚,马静,孙凌波,陈飞,张诗雨,张雨晗,李淼,张雅蓉,马红燕..基于双发射碳量子点的比率型荧光探针在Mn2+传感与细胞成像中的应用[J].中山大学学报（自然科学版）（中英文）,2025,64(3):60-73,14.基金项目
Supported by National Natural Science Foundation of China(22264023) （22264023）
Natural Science Foundation of Shaanxi Province(2024JC-YBQN-0150) （2024JC-YBQN-0150）
Yan'an Science and Technology Bureau Project(2023-SFGG-057) （2023-SFGG-057）
Scientific Research Projects of Education Department of Shaanxi Province(22JK0614) （22JK0614）
PhD Start Fund of Yan'an University(YDBK2022-15) （YDBK2022-15）
