智慧农业导刊2025，Vol.5Issue(10)：32-35,4.DOI:10.20028/j.zhnydk.2025.10.008
高校植物病毒可视化实验教学项目探索与实践
摘要
Abstract
This study aims to develop two plant virus visualization experiments using Loop-mediated Isothermal Amplification(LAMP)technology and fluorescent labeling techniques,providing new perspectives and practical methods for the reform of plant protection experimental teaching.After collecting plant samples suspected of tobacco mosaic viral infection,students extracted nucleic acids and processed the samples using LAMP technology.The presence of the target virus was determined by observing the color change in the reaction mixture.Additionally,students inoculated healthy Nicotiana benthamiana plants with TMV-GFP and monitoring viral trafficking using green fluorescent protein(GFP)labeling.The implementation of these two plant virus visualization experiments provided students with an intuitive learning experience.Not only did it broaden their horizons and stimulate their interest in learning,but it also enhanced their innovative spirit and practical abilities.关键词
植物病毒/实验教学/荧光标记技术/等温扩增技术/可视化实验项目Key words
plant viruses/experimental teaching/fluorescence labeling technology/isothermal amplification technology/visualization experimental items分类
社会科学引用本文复制引用
杨旸,李明骏,吴根土,窦彦霞,余钱,孙现超,青玲..高校植物病毒可视化实验教学项目探索与实践[J].智慧农业导刊,2025,5(10):32-35,4.基金项目
西南大学实验技术研究项目(SYJ2023039、SYJ2024037) （SYJ2023039、SYJ2024037）
重庆市教育委员会2024年高等教育教学改革研究项目(244013) （244013）
西南大学实验技术研究项目重点项目(SYJ2025010) （SYJ2025010）
