肿瘤防治研究2025，Vol.52Issue(5)：368-373,6.DOI:10.3971/j.issn.1000-8578.2025.24.0980
薯蓣皂苷元通过调节DAXX亚细胞定位激活JNK/p38信号通路诱导MCF-7细胞凋亡
Diosgenin Induces Apoptosis of MCF-7 Cells by Regulating DAXX Subcellular Localization and Activating JNK/p38 Signaling Pathway
摘要
Abstract
Objective To investigate the effect of diosgenin on the proliferation and apoptosis of breast cancer cells and its potential molecular mechanism.Methods The breast cancer cell line MCF-7 was treated with low,medium,and high doses of diosgenin,and cell proliferation was detected through the MMT method.Flow cytometry was used to detect cell apoptosis.Nuclear-cytoplasmic-protein separation method was applied to detect the subcellular localization of death associated protein(DAXX).qRT-PCR and Western blot were used to detect the expressions of DAXX and c-Jun N-terminal kinase pathway(JNK)-related proteins.Results Diosgenin considerably inhibited the proliferation of MCF-7 cells and promoted cell apoptosis in a concentration-dependent manner.Diosgenin can promote the movement of DAXX from nucleus into the cytoplasm.Diosgenin upregulated the expression of cell surface death receptor(Fas),increased the phosphorylation levels of JNK and mitogen activated protein kinase(p38),and activated the JNK/p38 signaling pathway with concentration dependence.Conclusion Diosgenin inhibits the proliferation and promotes the apoptosis of the breast cancer cell line MCF-7,whose mechanism may be related to the regulation of DAXX subcellular localization and the activation of JNK/p38 signaling pathway.关键词
乳腺癌/薯蓣皂苷元/DAXX/JNK/p38信号通路Key words
Breast cancer/Diosgenin/DAXX/JNK/p38 signaling pathway分类
临床医学引用本文复制引用
王佳,高世磊,张丽菡,张璐,孙旭,李华华,刘怀民..薯蓣皂苷元通过调节DAXX亚细胞定位激活JNK/p38信号通路诱导MCF-7细胞凋亡[J].肿瘤防治研究,2025,52(5):368-373,6.基金项目
National Natural Science Foundation of China(No.82205217) （No.82205217）
Clinical Scientist Special Project of Henan Province(No.S20240270) （No.S20240270）
Natural Science Foundation of Henan Province(No.222300420364) 国家自然科学基金(82205217) （No.222300420364）
河南省临床医学科学家培养专项(S20240270) （S20240270）
河南省自然科学基金(222300420364) （222300420364）
