肿瘤防治研究2025，Vol.52Issue(5)：374-381,8.DOI:10.3971/j.issn.1000-8578.2025.24.1072
TFAM通过调节代谢重编程对卵巢癌细胞化疗耐药的影响及其机制研究
Effect and Mechanism of TFAM on Chemotherapy Resistance in Ovarian Cancer Cells Through Metabolic Reprogramming Regulation
摘要
Abstract
Objective To investigate the role of mitochondrial transcription factor A(TFAM)in platinum-resistant ovarian cancer cells and its effects on metabolic reprogramming and sensitivity to platinum-based drugs Methods The mitochondrial function and metabolic characteristics of platinum-resistant ovarian cancer cells were analyzed.A TFAM-overexpressing cell model was established to assess its effects on platinum sensitivity,mitochondrial function,and aerobic glycolysis;and glycolytic enzyme and drug-resistant protein expression were analyzed.Results Platinum-resistant ovarian cancer cells exhibited considerable mitochondrial dysfunction(reduced oxygen consumption rate)and enhanced aerobic glycolysis(increased extracellular acidification rate,glucose uptake,and lactate production).TFAM was downregulated in resistant cells.Meanwhile,TFAM overexpression significantly enhanced platinum sensitivity(P<0.01),restored mitochondrial function,and inhibited aerobic glycolysis.The expression levels of glycolytic enzymes and drug-resistant proteins were also downregulated(P<0.05).Conclusion TFAM downregulation is associated with suppressed oxidative phosphorylation and enhanced aerobic glycolysis in platinum-resistant ovarian cancer cells.TFAM overexpression can restore cellular dependence on oxidative phosphorylation and increase platinum sensitivity,suggesting that TFAM is a potential therapeutic target for reversing platinum resistance.关键词
TFAM/有氧糖酵解/氧化磷酸化/卵巢癌/化疗耐药Key words
Mitochondrial transcription factor A/Aerobic glycolysis/Oxidative phosphorylation/Ovarian cancer/Chemotherapy resistance分类
医药卫生引用本文复制引用
范玲玲,热孜婉古丽·吾布力,卢玲岚,韩莉莉..TFAM通过调节代谢重编程对卵巢癌细胞化疗耐药的影响及其机制研究[J].肿瘤防治研究,2025,52(5):374-381,8.基金项目
People's Hospital of Xinjiang Uygur Autonomous Region Internal Project(No.20200408) 新疆维吾尔自治区人民医院院内项目(20200408) （No.20200408）
