中南民族大学学报（自然科学版）2025，Vol.44Issue(5)：616-628,13.DOI:10.20056/j.cnki.ZNMDZK.20250506
通过网络药理学探究宽筋藤治疗类风湿性关节炎的药理机制
Exploring the pharmacological mechanism of Tinospora sinensis on rheumatoid arthritis by a network pharmacology approach
摘要
Abstract
"Kuan-Jin-Teng"(TS)are used in traditional medicine for the treatment of rheumatoid arthritis.In this study,the possible pharmacological mechanism of anti-rheumatoid arthritis was investigated by using the method of network pharmacology.Finally,17 key targets corresponding to 22 active components of TS,which mainly involved three signaling pathways:Arachidonic acid metabolism pathway,PI3K-Akt signaling pathway and T cell receptor signaling pathway.In addition,through the construction and analysis of the"composition-target-pathway network",it is speculated that by regulating key proteins such as COX-2,LCK,P38 and ERK,the TS maintains the homeostasis of cytokine and arachidonic acid metabolism,so as to improve the pathological cellular immune disorders,and thus exert the therapeutic effect on RA.Finally,using RAW 264.7 mouse macrophages to conduct in vitro cell experiments and molecular docking simulations,compound C12 was found to reduce the biosynthesis of PGE2 by 52.8%,while compound C10 reduce the biosynthesis of LTB4 by 49.5%,suggesting that they could serve as potential COX-2 and ALOX5 inhibitors.The results are consistent with those predicted by the previous network pharmacological analysis,which verifies the rationality and reliability of the conclusions.关键词
类风湿性关节炎/宽筋藤/网络药理学/分子机制Key words
rheumatoid arthritis/Tinopspora sinensis(Lour.)Merr./network pharmacology/molecular mechanism分类
药学引用本文复制引用
王强,兰明,骆玺,蒙涛,杨光忠,李竣..通过网络药理学探究宽筋藤治疗类风湿性关节炎的药理机制[J].中南民族大学学报（自然科学版）,2025,44(5):616-628,13.基金项目
中央高校基本科研业务费专项资金资助项目(CZY18037) （CZY18037）
