中国现代医生2025，Vol.63Issue(15)：56-60,5.DOI:10.3969/j.issn.1673-9701.2025.15.012
益肾升阳方经Bach2 调控Treg细胞治疗变应性鼻炎
Yishen Shengyang formula ameliorates allergic rhinitis via Bach2-driven Treg cell regulation
摘要
Abstract
Objective To investigate the therapeutic effects and mechanisms of Yishen Shengyang formula in allergic rhinitis(AR)by regulating the transcription factor BTB and CNC homology 2(Bach2)to promote regulatory T cell(Treg cell)differentiation.Methods AR mouse models were established via ovalbumin(OVA)sensitization and randomly divided into control group,AR model group(AR group),Yishen Shengyang formula group and dexamethasone group.Nasal symptoms were evaluated using behavioral scores;Histopathological changes in nasal mucosa were observed via hematoxylin-eosin and periodic acid Schiff stain;Serum levels of OVA-specific immunoglobulin E(sIgE),interleukin-4(IL-4),and transforming growth factor-β(TGF-β)were measured by enzyme linked immunosorbent assay;Splenic Treg proportions were analyzed via flow cytometry;Bach2 protein expression was assessed by Western blot.Results Compared with control group,AR group mice showed significantly increased behavioral scores(P<0.05),goblet cell hyperplasia,inflammatory infiltration,elevated OVA-sIgE and IL-4 levels(P<0.05),reduced TGF-β(P<0.05),and decreased splenic Treg proportions and Bach2 expression(P<0.05).Yishen Shengyang formula treatment alleviated nasal symptoms(P<0.05),mitigated mucosal damage,decreased OVA-sIgE and IL-4,increased TGF-β(P<0.05),and upregulated Treg proportions and Bach2 expression(P<0.05),with efficacy comparable to dexamethasone.Conclusion Yishen Shengyang formula demonstrates potential therapeutic effects on allergic rhinitis by upregulating Bach2 expression and promoting Treg cell differentiation.This study provides a novel strategy and experimental basis for the traditional Chinese medicine treatment of AR.关键词
益肾升阳方/变应性鼻炎/Bach2/调节性T细胞/免疫调控Key words
Yishen Shengyang formula/Allergic rhinitis/Bach2/Regulatory T cell/Immune regulation分类
临床医学引用本文复制引用
马志超,张朝晖,应永杰,王艺龙,金巧智,陈武兵,陶宝鸿..益肾升阳方经Bach2 调控Treg细胞治疗变应性鼻炎[J].中国现代医生,2025,63(15):56-60,5.基金项目
浙江省中医药科技计划项目(2023ZL196) （2023ZL196）
