广东海洋大学学报2025，Vol.45Issue(2)：27-34,8.DOI:10.3969/j.issn.1673-9159.2025.02.004
脯氨酸对高盐下缢蛏鳃组织结构、抗氧化能力及细胞凋亡的影响
Effects of Proline on Gill Tissue Structure,Antioxidant Capacity and Apoptosis of Sinonovacula constricta under High Salt Stress
摘要
Abstract
[Objective]To investigate the effects of proline on the gill tissue structure,antioxidant capacity and apoptosis of Sinonovacula constricta under high salt stress,and analyze the regulation mechanism of high salt tolerance.[Method]S.constricta with shell length(4.7±0.3)cm and body mass(5.0±0.3)g were cultured in salinity 20(the control),salinity 32(group PS0),and salinity 32 water supplemented with 5.0,7.5,and 10.0 μg/mL proline(recorded as groups PS5,PS7.5,and PS10,respectively)for 96 h.Gill tissue sections were observed,antioxidant enzyme activity were detected,and apoptosiswere detect by TUNEL.[Result]Under the condition of high salt stress,the gill tissue structure of group PS0 changed significantly,with the prolongation of the stress time,such asgill filament dissolution,gill lumen expansion and severe overall contraction.The gill tissue structure of group PS5 was alleviated compared to that of the high-salt group,and there was no significant change in the overall gill tissue structure,gill organization was more complete in groups PS7.5 and PS10,the gill tissue structure was more intact at 0 to 48 h.At 72 h,the gill lumen showed signs of enlargement;at 96 h,the gill lumen was enlarged even more and the gill filaments were wrinkled.Oxidative stress in gill tissues due to high salt stress,MDA content in group PS0 was significantly lower at 12 h and significantly higher thereafter(P<0.05),the activity of SOD was significantly elevated,reaching a maximum at 24 h and then falling back,and remained significantly higher than that of the control at 96 h(P<0.05),and the activity of CAT was significantly decreased,falling to a minimum at 12 h and then rebounding,and remained significantly lower than that of the control at 96 h(P<0.05).Compared with the high-salt group,the MDA content of each proline-added group was significantly reduced at 24 to 96 h(P<0.05);the SOD activity of each proline-added group decreased and then increased,and the SOD activity of groups PS5,PS7.5,and PS10 decreased significantly at 12 to 24 h,6 to 12 h,and 12 h,respectively,and then gradually recovered,and was significantly higher than that of the high-salt group at 72 to 96 h(P<0.05);the CAT activity of group PS5 increased significantly at all time points except 24 h,and the CAT activity of the PS7.5 and PS10 groups was significantly higher than that of the high-salt group only at 72 to 96 h(P<0.05);there was no significant difference in the three antioxidant indexes among the proline-added groups at 96 h(P>0.05).Under high salt stress,the apoptotic signal of gill cells gradually increased,and a large number of gill filament cells showed all apoptotic signals.But there was nosignificant apoptotic signal in group PS5.Groups PS7.5 and PS10 showed no significant apoptotic signal at 0 to 48 h,but at 72 h,the apoptotic signal showed signs of increase,and at 96 h,significant increase in apoptotic signaling.[Conclusion]Proline can effectively alleviate tissue damage and oxidative stress caused by high salt stress,reduce cell apoptosis,and thus improve the body's tolerance.Adding 5.0 μg/mL proline has a better alleviating effect on high salt stress.关键词
缢蛏/盐度/脯氨酸/鳃组织结构/抗氧化/细胞凋亡Key words
Sinonovacula constricta/salty/proline/gill microstructure/antioxidation/apoptosis分类
水产学引用本文复制引用
张娜,刘瑞琦,詹天宇,金凯,牛东红..脯氨酸对高盐下缢蛏鳃组织结构、抗氧化能力及细胞凋亡的影响[J].广东海洋大学学报,2025,45(2):27-34,8.基金项目
国家自然科学基金(32373118) （32373118）
