安徽农业科学2025，Vol.53Issue(10)：103-110,8.DOI:10.3969/j.issn.0517-6611.2025.10.020
陆地棉GhHB51基因克隆·生物信息学分析及遗传转化
Cloning,Bioinformatics Analysis and Genetic Transformation of GhHB51 Gene in Gossypium hirsutum
摘要
Abstract
To study the potential function of GhHB51 gene in the growth and development of Gossypium hirsutum,and cultivate transgenic cot-ton,so as to lay the foundation for further study of GhHB51 gene function and screening of new excellent cotton germplasm.GhHB51 gene was cloned using the genetic standard line"TM-1"of Gossypium hirsutum,and bioinformatics,transcriptome data and tissue specificity analysis were performed.The gene editing vector was constructed and used to transform cotton by Agrobacterium tumefacion-mediated method.The coding region of the gene was 534 bp,encoding 177 amino acids,containing Homedomain and HALZ domains.The promoter region contains several cis-regulatory elements related to stress response and growth and development,which are significantly expressed under drought stress.The gene editing vector was constructed and successfully transformed into cotton,and 12 transgenic plants were obtained.关键词
棉花/GhHB51基因/生物信息学分析/遗传转化Key words
Cotton/GhHB51 genes/Bioinformatics analysis/Genetic transformation分类
农业科技引用本文复制引用
黄秦,左东云,王巧连,吕丽敏,程海亮,陈全家,宋国立..陆地棉GhHB51基因克隆·生物信息学分析及遗传转化[J].安徽农业科学,2025,53(10):103-110,8.基金项目
国家棉花产业体系(CARS-15-06) （CARS-15-06）
科技创新2030重大项目"早熟抗病棉花新品种设计与培育"(2023ZD04041). （2023ZD04041）
