滨州医学院学报2025，Vol.48Issue(2)：125-129,5.DOI:10.19739/j.cnki.issn1001-9510.2025.02.003
细胞爬片式共培养模型的建立和优点
Establishment and advantages of cell crawing sheets co-culture model
摘要
Abstract
Objective To investigate the effects of human lung adenocarcinoma A549 cells in different co-culture modes on the phenotype and function of human umbilical vein endothelial fusion cells EA.hy.926 cells.Methods The co-culture models of A549 cells and EA.hy.926 cells were established by "cell creep" co-culture method and "transwell chamber" co-culture method,respectively,and the integrity of endothelial cell barrier was evaluated by FITC-glucan cell penetration test.The pheno-type and functional changes of EA.hy.926 cells were detected by Western blot analysis of polysaccharide coated core proteins SDC-1,tight junction proteins ZO-1,occludin-related proteins and CCK8 detection of endothelial cell proliferation activity.Re-sults Compared with transwell cell co-culture model,in the FITC-glucan cell penetration test,the infiltration rate of EA.hy.926 cells was significantly increased by cell creep co-culture model(P＜0.05),indicating that endothelial cell integrity was damaged and cell permeability was increased;by Western blot,the cell creep co-culture model significantly downregulated the expression of SDC-1,ZO-1 and Occludin of EA.hy.926 cells,and the damage was obvious(P＜0.05);by CCK8 experiment,the prolifer-ation activity of EA.hy.926 cells was significantly increased in the creep co-culture model(P＜0.05).Conclusion Compared with transwell cell co-culture model,the cell creep co-culture model can better reflect the influence of tumor cells on endothelial cells,providing a new research model for the study of lung cancer metastasis.关键词
肺癌/内皮细胞/多配体蛋白聚糖-1/细胞连接/细胞骨架/细胞共培养Key words
lung cancer/EA.hy.926/syndecan-1/cell junction/cytoskeleton/cell co-culture分类
医药卫生引用本文复制引用
陈阿惠,李涛,董鑫鑫,翟乃亮..细胞爬片式共培养模型的建立和优点[J].滨州医学院学报,2025,48(2):125-129,5.基金项目
国家自然科学基金青年基金(82200082) （82200082）
山东省医药卫生科技发展计划(2018WS546) （2018WS546）
