中国耳鼻咽喉头颈外科2025，Vol.32Issue(4)：234-238,5.DOI:10.16066/j.1672-7002.2025.04.007
miR-21-5p在鼻黏膜2型炎症中的表达及下游调控通路研究
The expression and downstream pathways of miR-21-5p in nasal type 2 inflammation
摘要
Abstract
OBJECTIVE To explore the expression,regulation,and downstream pathways of miR-21-5p in nasal type 2 inflammatory diseases.METHODS The expression levels of miR-21-5p were evaluated in nasal mucosa of patients with chronic rhinosinusitis with nasal polyps(CRSwNP)and healthy controls,as well as in nasal mucosa of CRSwNP and allergic rhinitis(AR)mouse models by real-time PCR.Human primary nasal epithelial cells were cultured and treated with different cytokines.The expression of miR-21-5p were then detected after 48 hours treatment.miR-21-5p lentivirus were used for overexpression in nasal epithelial cells.After 72 hours,cells were collected,and RNA was extracted for transcriptome analysis.Differentially expressed genes were further analyzed for pathway enrichment.RESULTS Compared with healthy controls,the expression of miR-21-5p was significantly increased in the nasal mucosa of CRSwNP patients(P<0.01).The expression of miR-21-5p was also significantly increased in mouse models of CRSwNP and AR respectively,compared to the control groups(P<0.01,respectively).The type 2 inflammation cytokines IL-4 and IL-13 can significantly upregulate miR-21-5p expression in nasal epithelial cells(P<0.01,respectively).Overexpression by lentivirus can significantly increase the expression of miR-21-5p in nasal epithelial cells,and lead to 118 differentially expressed genes.Those genes are significantly enriched in pathways associated with posttranscriptional gene silencing,WNT signaling and MAPKinase signaling.CONCLUSION miR-21-5p is upregulated in nasal type 2 inflammatory diseases partially controlled by IL-4 and IL-13.Overexpression of miR-21-5p lead to the changes of downstream pathways associated with WNT signaling and MAPKinase signaling in nasal epithelial cells.关键词
鼻窦炎/鼻息肉/鼻炎,过敏/2型炎症/miR-21-5pKey words
Sinusitis/Nasal Polyps/Rhinitis,Allergic/type 2 inflammation/miR-21-5p引用本文复制引用
王明,栾格,袁婧,王成硕,张罗..miR-21-5p在鼻黏膜2型炎症中的表达及下游调控通路研究[J].中国耳鼻咽喉头颈外科,2025,32(4):234-238,5.基金项目
国家自然科学基金项目(82471138、82171109、81800882) （82471138、82171109、81800882）
