医学分子生物学杂志2025，Vol.22Issue(3)：211-216,6.DOI:10.3870/j.issn.1672-8009.2025.03.001
二氢石蒜碱对CVB3诱导的病毒性心肌炎大鼠的保护作用分析
Protective Effect of Dihydrolycorine on Rats with CVB3-induced Viral Myocarditis
摘要
Abstract
Objective To explore the influence and mechanism of dihydrolycorine(DL)on rats with viral myocarditis(VMC)induced by Coxsackie B3 virus(CVB3).Methods A total of 72 SD rats were randomized into 6 groups:blank control group,model group,10 mg/kg DL group,20 mg/kg DL group,40 mg/kg DL group,and Shenmai injection group(200 mg/kg),with 12 rats in each group.The CVB3-induced VMC model was established and intervened for 7 days.The levels of serum myocardial enzymes and the hemodynamic indexes were detected.HE stai-ning was used to detect the myocardial tissue pathological damage.TUNEL was used to detect myo-cardial cell apoptosis.Western blotting was applied to detect the expression levels of apoptosis and endoplasmic reticulum stress-related proteins.Results Compared with those in the model group,the pathological damage of myocardial tissue was alleviated,and the levels of serum myoglobin(Mb),creatine kinase isoenzyme MB(CK-MB)and cardiac troponin Ⅰ(cTnⅠ),the myocardial apoptosis rate and the protein expression levels of BCL2-associated X protein(Bax),C/EBP ho-mologous protein(CHOP),glucose-regulated protein 78(GRP78)and GRP94 were reduced in the 20 and 40 mg/kg DL groups and the Shenmai injection group(P＜0.05).Moreover,the left ventricular pressure(LVP),the maximum change rate of left ventricular pressure rise and fall(±dp/dtmax),and the protein expression levels of B-cell lymphoma-2(Bcl-2)and the cleaved/caspase-3 ratio rose in the 20 and 40 mg/kg DL groups and the Shenmai injection group(P＜0.05).Conclusion DL can relieve the VMC myocardial injury and improve the cardiac dysfunc-tion,which may be related to the inhibition of endoplasmic reticulum stress-mediated apoptosis.关键词
二氢石蒜碱/内质网应激/病毒性心肌炎/心肌损伤/柯萨奇B3病毒/细胞凋亡Key words
dihydrolycorine/endoplasmic reticulum stress/viral myocarditis/myocardial in-jury/coxsackie B3 virus/apoptosis分类
药学引用本文复制引用
白红敏,杨永忠,陈树杰,杨超,刘伟..二氢石蒜碱对CVB3诱导的病毒性心肌炎大鼠的保护作用分析[J].医学分子生物学杂志,2025,22(3):211-216,6.基金项目
河北省医学科学研究课题(No.20220550) This work was supported by a grant from the Medical Science Research Project of Hebei Province(No.20220550) （No.20220550）
