医学分子生物学杂志2025，Vol.22Issue(3)：217-222,6.DOI:10.3870/j.issn.1672-8009.2025.03.002
奥拉帕尼对卵巢癌ES2细胞生存、上皮细胞间质转换及肿瘤干细胞样特性的调节作用
Effect of Olaparib on Survival,Epithelial-mesenchymal Transition,and Tumor Stem Cell-like Characteristics in Ovarian Cancer ES2 Cells
摘要
Abstract
Objective To explore the effect of olaparib on the survival,epithelial-mesenchy-mal transition(EMT)and tumor stem cell-like characteristics of ovarian cancer ES2 cells.Methods Ovarian cancer ES2 cells were culturedin vitro,and they were divided into 5 groups:control group,low-dose olaparib group,middle-dose olaparib group,high-dose olaparib group and doxorubicin 0.5 μmol/L group.Cells proliferation,apoptosis,invasion and migration were detected by 5-ethynyl-2'-deoxyuridine(EDU)staining,flow cytometry,Transwell assay,and wound healing assay,respectively.The epithelial-mesenchymal transition(EMT)of cells was observed under light microscope,tumor stem cell-like characteristics were observed by tumor sphe-roid formation assay,and protein expression level was detected by Western blotting.Results Com-pared with those in the control group,the apoptosis rate of ES2 cells and the expression level of E-cadherin were significantly increased in the low-,middle-,high-dose olaparib group and doxorubi-cin 0.5 μmol/L group(P＜0.05),the ratio of EDU staining positive cells,number of invasion cells per unit area,cell wound-healing rate,number of tumor spheroid,spheroid diameter,and the expression levels of nuclear antigen Ki67,Survivin,vascular endothelial growth factor(VEGF),N-cadherin,cluster of differentiation 44(CD44)and aldehyde dehydrogenase 1(AL-DH1)were significantly decreased in the middle-and high-dose olaparib group and doxorubicin 0.5μmol/L group(P＜0.05).Conclusion Olaparib of over 30 mg/L can significantly inhibit prolifer-ation,invasion,and migration of ovarian cancer ES2 cells,and promote their apoptosis,which may be through inhibition of Ki67 and regulation of EMT.关键词
卵巢癌/聚腺苷二磷酸核糖聚合酶/奥拉帕尼/细胞凋亡/细胞迁移Key words
ovarian cancer/poly(ADP-ribose)polymerase/olaparib/apoptosis/cell mi-gration分类
药学引用本文复制引用
叶晶,赵金荣,李萍,韩慈,张欣,程伟,田晓茹..奥拉帕尼对卵巢癌ES2细胞生存、上皮细胞间质转换及肿瘤干细胞样特性的调节作用[J].医学分子生物学杂志,2025,22(3):217-222,6.基金项目
河北省卫生健康委科研基金项目(No.20191730),河北省2022年度医学科学研究课题(No.20221905) This work was supported by grants from the Scientific Research Fund Project of Hebei Provincial Health Commission(No.20191730),and the Hebei Province 2022 Annual Medical Science Research Project(No.20221905) （No.20191730）
